Dataset: carbonate_chemistry
Deployment: lab_Carpenter_Moorea

Various experimental carbonate chemistry conditions used to observe effects of acidification on corals and calcified algae

Co-Principal Investigator:

Robert Carpenter (California State University Northridge, CSUN)

Peter J. Edmunds (California State University Northridge, CSUN)

Contact:

Steeve Comeau (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Danie Kinkade (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

RUI: Ocean Acidification- Category 1- The effects of ocean acidification on the organismic biology and community ecology of corals, calcified algae, and coral reefs (OA_Corals)

Version:

2012-12-05

Version Date:

2012-12-05

Data URL:

https://www.bco-dmo.org/dataset-deployment/455567/data

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Description

Carbonate chemistry components of various seawater treatments used during experiments involving the coral Porites rus and alga Hydrolithon onkodes. These data are part of a larger data set containing light, dark, and mean calcification rates, which in turn are part of experiments performed during July-August 2011 in French Polynesia to test the effect of various concentrations of carbonate and bicarbonate ions on the calcification of a coral and a calcifying alga.

Related References:

- Comeau, S., Carpenter, R. C., & Edmunds, P. J. (2013). Coral reef calcifiers buffer their response to ocean acidification using both bicarbonate and carbonate. Proceedings of the Royal Society B: Biological Sciences, 280(1753), 20122374. doi:10.1098/rspb.2012.2374

Related datasets:

algae_calcification
coral_calcification
light_dark_calcification
mean_calcification
calcification rates - flume expt
carbonate chemistry - flume expt

Methods & Sampling

Dataset acquisition description

Seawater pH was measured once or twice a day (08:00 hrs and 18:00 hrs) in each incubation tank, using a pH meter (Orion, 3-stars mobile) or an automatic titrator (T50, Mettler-Toledo) calibrated every 2-3 d on the total scale using Tris/HCl buffers (Dickson, San Diego, USA) with a salinity of 34.5. Total alkalinity of the seawater in the tanks was measured every 2 d following water changes, using single samples drawn from each tank in glass-stoppered bottles (250 mL). Samples were analyzed for total alkalinity within 1 d using open cell, potentiometric titration and an automatic titrator (T50, Mettler-Toledo). Titrations were conducted on 50-mL samples at ~ 24 °C, and total alkalinity calculated as described by Dickson et al. [2007]. Titrations of certified reference materials (CRM) provided by A. G. Dickson (batch 105) yielded total alkalinity values within 4.0 µmol kg-1 of the nominal value (SD = 4.1 µmol kg-1; n = 12). Salinity in the experimental tanks was measured every 2 d using a conductivity meter (YSI 3100). Total alkalinity, pH on the total scale (pHT), temperature, and salinity were used to calculate [HCO3-] and [CO32-] using the seacarb package (Lavigne and Gattuso 2011) running in R software (R Foundation for Statistical Computing).

Data Processing Description

Dataset Processing Description

BCO-DMO Processing Notes:

Original data file: “Raw_data_Comeau_et_al_PRSB-1.xls” contained three spreadsheets. This file was split into three separate datasets. Resultant datasets were edited in excel by BCODMO. The following edits were made to the file carbonate_chemistry.dat:

-Added BCO-DMO header
    -edited 'Date' to 'date_local', removed alphanumeric 'date_local' from final view
    -edited 'Dissolved Inorganic Carbon' to 'DIC'
    -edited 'Total Alkalinity' to 'TALK'
    -edited 'Temperature' to 'temp'
    -replaced spaces in condition text with underscores
-Added 'day_local', 'month_local', 'year' to served view
-Added 'lat' and 'lon' column of experiment site (Richard B. Gump Research Site, Moorea) to file
-Added title, version and PI lines

-2012.12.07 Data contact S. Comeau submitted an updated file with corrected dates.

-2013.09.29 Header parameter names edited/corrected as per S. Comeau.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1041270	NSF Division of Ocean Sciences

Instruments

Automatic titrator

Supplied Name: automatic titrator

Supplied Description:

A Mettler-Toledo T50 model automatic titrator was employed to analyze samples for total alkalinity using open cell, potentiometric titration.

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Benchtop pH Meter

Supplied Description:

An Orion, 3-star mobile pH meter was used to measure pH in each incubation tank.

Instrument Type

Generic Name: Benchtop pH Meter

Generic Description:

An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms)

This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
CO3	Carbonate ion concentration.	mol/kg	no_bcodmo_term
condition	Experimental treatment of various concentrations of carbonate and bicarbonate ions used to test the effects of various carbonate chemistry regimes on coral and calcifying alga.	dimensionless	no_bcodmo_term
day_local	Two-digit day in local time.	dd	day_local
deploy_id	Unique descriptor for experiments, indicating physical location where they were conducted (lab), lead investigator and lab location.	dimensionless	no_bcodmo_term
DIC	Dissolved inorganic carbon.	mol/kg	DIC
HCO3	Bicarbonate ion concentration.	mol/kg	no_bcodmo_term
lat	Latitude component of geographic position where experiments were conducted.	decimal degrees	lat
lon	Longitude component of geographic position where experiments were conducted.	decimal degrees	lon
month_local	Two-digit month of the year in local time.	mm	month_local
pCO2	Partial pressure of CO2 in seawater.	micro atmospheres	no_bcodmo_term
pH	Measure of the acidity of a solution based upon the log of the hydrogen ion concentration.	dimensionless; pH scale	pH
TALK	Total Alkalinity; a measure of the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate, equal to the sum of the bases in solution.	mol/kg	TALK
temp	Water temperature.	degrees Celsius	temp
year	Four-digit year.	yyyy	year
yrday_local	Day of year in local time.	ddd	yrday_local

Database

Contribute Data

Dataset: carbonate_chemistry
Deployment: lab_Carpenter_Moorea

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: carbonate_chemistryDeployment: lab_Carpenter_Moorea

Dataset acquisition description

Dataset Processing Description

Dataset: carbonate_chemistry
Deployment: lab_Carpenter_Moorea