Dataset: Sediment chlorophyll and phaeophytin
Deployment: Spivak_2012

Raw benthic chlorophyll and phaeophytin data.

Principal Investigator:

Amanda Spivak (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Hannah Ake (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Eutrophication Effects on Sediment Metabolism and Benthic Algal-bacterial Coupling: An Application of Novel Techniques in a LTER Estuary (Benthic_PP_at_TIDE)

Current State:

Final no updates expected

Version:

Final

Version Date:

2016-12-08

Data URL:

https://www.bco-dmo.org/dataset-deployment/669657/data

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Description

Raw benthic chlorophyll and phaeophytin data from each experimental time point in June, August, and October 2013.

Methods & Sampling

Dataset acquisition description

Sediment samples for organic matter composition were collected by placing a hard plastic sleeve around a polyvinyl chloride (PVC) corer (5 cm diameter x 15 cm deep) and then removing the corer. The plastic sleeve remained in place to maintain the integrity of the sediment column and mark the core location (Spivak 2015). The top 0.5 cm of each core was collected into pre-combusted vials and frozen (-80 deg C) until analysis for total organic carbon and nitrogen content and stable isotopes (d13C, d15N) and lipid biomarker composition. Adjacent samples for benthic chlorophyll were collected with smaller cores (1.5 cm diameter x 1 cm deep) into glass vials and frozen (-20 deg C) until analysis. Additional sediment cores for organic matter composition and benthic chlorophyll were collected 4, 8, 24, and 48 h after the 13C-labeled NaHCO3 was applied in June, August, and October and 4, 8, 24, and 144h after the 13C-labeled S. alterniflora was applied in August. Benthic chlorophyll was determined per methods described by Neubauer et al. (2000).

References:

Spivak, AC and J Ossolinski. 2016. Limited effects of nutrient enrichment on bacterial carbon sources in salt marsh tidal creek sediments. Marine Ecology Progress Series. 544:107-130.10.3354/meps11587

Data Processing Description

Dataset Processing Description

The file includes raw and log10-transformed benthic chlorophyll and phaeophytin data.

BCO-DMO Data Processing Notes:

-reformatted column names to comply with BCO-DMO standards.
-displayed months numerically

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1233678	NSF Division of Ocean Sciences

Instruments

Push Corer

Supplied Name: Core

Supplied Description:

Used to collect core samples

Instrument Type

Generic Name: Push Corer

Generic Description:

Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.

Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
month	Samping month; mm	unitless	month
plot	Plot ID	unitless	site
estuary	Estuary where sampling occurred	unitless	site
timepoint	Timepoint refers to when the sample was collected before (PL) or after the 13C-isotope label was added	unitless	time
experiment	Experiment refers to whether the 13C label was applied as benthic microalgae (BMA) or Spartina alterniflora (salt) detritus.	unitless	treatment
sampleID	Sample ID number	unitless	sample
chla	Benthic chlorophyll concentration	ug cm-2	chl_a
phaeo	Phaeophytin concentration	ug cm-2	phaeo
logchla	Log 10 benthic chlorophyll concentration	log	chl_a
logphaeo	Log 10 phaeophytin concentration	log	phaeo
chla_phaeo	Ratio of chlorphyll to phaeophytin concentrations	ug cm-2	Fo_to_Fa

Database

Contribute Data

Dataset: Sediment chlorophyll and phaeophytin
Deployment: Spivak_2012

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Sediment chlorophyll and phaeophytinDeployment: Spivak_2012

Dataset acquisition description

Dataset Processing Description

Dataset: Sediment chlorophyll and phaeophytin
Deployment: Spivak_2012