Dataset: Synechococcus Si uptake: phosphorous
Deployment: Krause_DISL_2013-2015

Monocultures of four Synechococcus clones (1333, 1334, 2515, 2370)  were used to examine variation and controls on Si quotas and rates of Si accumulation.  Cultures were procured from the National Center for Marine Algae and Microbiota (NCMA) at the Bigelow Laboratory for Ocean Sciences in Boothbay Harbor, Maine.  Many of these clones are also available in other culture collections and have various strain names; here we will refer to each by their NCMA strain number (a.k.a. CCMP number).  

Cultures were kept in surface Sargasso seawater (ambient concentrations ~1 μM Si(OH)4, ~0.1 uM PO4)  with added orthophosphate (f/2 level, 36 μM) as well as the same seawater without added orthophosphate.  

Each incubation was terminated by filtration and processed for measurement of 32Si activity following Krause et al. (2011).   After aging for 120 days, secular equilibrium between 32Si and its daughter 32P was achieved and 32Si activity was determined using gas-flow proportional counting using GM 25-5 multicounters (Risø National Laboratory, Technical University of Denmark).  

Full details of culturing and experimental methods are described in Brzezinski et al. (in review). (as of 05 Jan 2017)