Instrument: Raman Microscope
The Raman microscope is a laser-based microscopic device used to perform Raman spectroscopy. The Raman microscope begins with a standard optical microscope, and adds an excitation laser, laser rejection filters, a spectrometer or monochromator, and an optical sensitive detector such as a charge-coupled device (CCD), or photomultiplier tube, (PMT). One example is the XploRA confocal Raman microscope (information from the manufacturer).
Raman spectra were acquired with an XploRA confocal Raman microscope by Horiba Jobin Yvon, Inc. The system includes a flat field spectrograph with a multichannel air cooled CCD detector and color camera optically coupled to an Olympus BX41 microscope. The investigators used three lasers: a 532 nm 25 mW solid-state laser, a 638 nm 25 mW laser diode, and a 785 nm 25 mW laser diode. See more information about the Raman microscope on the UMCES website.