Instrument: Flow Cytometer
Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
Approximately 10,000 0.75 m beads (FluoresbriteTM particles, YG, Polysciences, Inc.) were added into each sample and samples were analyzed by flow-cytometer (BD Influx cell sorter) triggering on forward angle light scatter (FSC) to determine total cell numbers of populations of Prochlorococcus, Synechococcus and pico-eukaryotic algae.