Instrument: CytoBuoy CytoSense flow cytometer

Dataset: 
Instrument Short Name: CytoSense
Instrument Description:

The CytoSense is a portable, benchtop autonomous flow cytometer designed for phytoplankton species classification and analysis of filamentous algae. It can also be used in situ to reveal temporal and spatial phytoplankton variability. It can be remotely controlled, and has been specifically designed to record the optical pulse shapes of suspended particles between <1 and 800 micrometres in diameter and up to 4 millimetres in length (for chain-forming cells recording) in relatively large volumes of water (several centimetres cubed per sample). The instrument combines high sensitivity with an extremely wide particle size range (from sub-micron up to 1.5 millimetres in diameter) and acquires multiple data points per particle, which distinguishes the CytoSense from conventional flow cytometers. The CytoSense has a modular design, with various upgrades and accessories available to suit user requirements. These include additional lasers, optional cameras to take pictures of particles and a widened flow cell. The sample intake speed ranges from 0.07 - 17 microlitres per second, allowing high particle loads (thousands of particles per second) as well as very low concentrations.

Alternate name: CytoBuoy CytoSense flow cytometer

PI supplied instrument name: CytoSense benchtop flow cytometer
Dataset-specific description

CytoSense benchtop flow cytometer

Since 2012, a variety of flow cytometric instruments are utilized to quantify picoplankton and larger microplankton including diatoms and dinoflagellates in our SRiMP program. Picoplankton analysis is performed by Dr. Liz Mann using a FacsCalibur (BD Biosciences, San Jose, CA, USA) in house. Additionally, we employ a state of the art CytoSense benchtop flow cytometer equipped with an imaging system and wide (1.5 mm) flow-cell uniquely capable of rapid analysis of live cells including large and chain-forming species (up to 4 mm long) such as diatoms and dinoflagellates (CytoBouy b.v., Woerden, the Netherlands, http://www.cytobuoy.com) performed in house by Dr. Jens Nejstgaard.