Instrument: Fluorescence Microscope Image Analysis System
A Fluorescence (or Epifluorescence) Microscope Image Analysis System uses semi-automated color image analysis to determine cell abundance, dimensions and biovolumes from an Epifluorescence Microscope. An Epifluorescence Microscope (conventional and inverted) includes a camera system that generates enlarged images of prepared samples. The microscope uses excitation ultraviolet light and the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light.
The organisms were counted using a ZEISS Axioplan Epifluorescence Microscope equipped with a 100x Plan-NEOFLUAR 100x/1.30 oil, objective lens