Instrument: PCR Thermal Cycler

Instrument Short Name: Thermal Cycler
Instrument Description:

A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.

(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)

PI supplied instrument name: Thermocycler
Dataset-specific description

Loci were amplified on a thermocycler (BioRad). The PCR program included 2 min at 95 degrees C, 30 cycles of 30 s at 95 degrees C, 30 s at 55 degrees C and 30 s at 72 degrees C, and a final 5 min at 72 degrees C.