Scheduled Service Outage - Oct. 23 (9 - noon ET)

Please be aware that on Monday, October 23rd, BCO-DMO will conduct a scheduled outage on all systems and services between 9AM & noon ET. For more information, see the full notice.

Instrument: Automated DNA Sequencer

Instrument Short Name: Automated Sequencer
Instrument Description:

General term for a laboratory instrument used for deciphering the order of bases in a strand of DNA. Sanger sequencers detect fluorescence from different dyes that are used to identify the A, C, G, and T extension reactions. Contemporary or Pyrosequencer methods are based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step.

PI supplied instrument name:
Dataset-specific description

Big Dye Terminator 3.1 Cycle Sequencing chemistry at the High-Throughput Genomics Unit, University of Washington or on an ABI PrismTM 377 DNA Sequencer (Applied Biosystems).