Dataset: 2018 Salt Pond

Name: Environmental observations and infected host and dinospore counts in Salt Pond, Falmouth, Massachusetts, USA from 2018 through 2019
Published: 2020-03-10
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.805612.1

Spatial Extent: N:41.54482 E:-70.62723 S:41.54482 W:-70.62723

Temporal Extent: 2018-03-19 - 2019-08-09

Project:

Trojan Horses in the Marine Realm: Protist Parasite-host Dynamics in Coastal Waters (Coastal Parasites)

Principal Investigator:

Rebecca J. Gast (Woods Hole Oceanographic Institution, WHOI)

Co-Principal Investigator:

Virginia P. Edgcomb (Woods Hole Oceanographic Institution, WHOI)

Maria G. Pachiadaki (Woods Hole Oceanographic Institution, WHOI)

Contact:

Virginia P. Edgcomb (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2020-03-10

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Environmental observations and infected host and dinospore counts in Salt Pond, Falmouth, Massachusetts, USA from 2018 through 2019

Abstract:

Salt Pond is a shallow, seasonally stratified coastal salt pond located in Falmouth, Massachusetts, USA and it is proximal to the labs at Woods Hole Oceanographic Institution (WHOI). This estuarine system is tidally influenced by Vineyard Sound and receives inputs of freshwater primarily from groundwater. Seasonal abiotic factors and nutrient availability in the seasonally stratified water column influence the growth and abundance of diverse microbial taxa within this system, providing an ideal setting to study syndinian parasites and host diversity temporally and across oxygen gradients. Salt Pond has anoxic waters that come within 2-3 meters of the water surface in summer and bottom waters that become increasingly sulfidic due to bacterial sulfate reduction as seasonal stratification intensifies.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2020-03-10	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.805612.1	2023-03-20

Description

Salt Pond is a shallow, seasonally stratified coastal salt pond located in Falmouth, MA and it is proximal to the labs at Woods Hole Oceanographic Institution (WHOI). This estuarine system is tidally influenced by Vineyard Sound and receives inputs of freshwater primarily from groundwater. Seasonal abiotic factors and nutrient availability in the seasonally stratified water column influence the growth and abundance of diverse microbial taxa within this system, providing an ideal setting to study syndinian parasites and host diversity temporally and across oxygen gradients. Salt Pond has anoxic waters that come within 2-3 meters of the water surface in summer and bottom waters that become increasingly sulfidic due to bacterial sulfate reduction as seasonal stratification intensifies.

Methods & Sampling

For the field sampling protocol, please see the attached Supplemental File FieldSamplingProtocol_2018_BCO-DMO.docx.

For information concerning the in-situ hybridization, please see the Supplemental Files CARD-FISH Protocol.pdf and Reagents for CARD-FISH .pdf.

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- added lat and lon fields
- reformatted date to yyyy-mm-dd
- joined environmental, infection, and spore count datasets on the sample date, cast number, and depth fields.

Data Files

File
combined.csv (Comma Separated Values (.csv), 49.83 KB) MD5:47da7c0ecc5d4eec8f7f34a03f816d3d Primary data file for dataset ID 805612

Supplemental Files

File
CARD-FISH Protocol for dataset 805612 filename: CARD-FISH_Protocol.pdf (Portable Document Format (.pdf), 171.08 KB) MD5:59c1e7dbbe753debbd1a62f2eb161b43 Contains information relevant to accomplishing in situ hybridization.
Field Sampling Protocol for dataset 805612 filename: FieldSamplingProtocol_2018_BCO-DMO.docx (Octet Stream, 14.22 KB) MD5:842829fb66c6c6440437d4c193843591 Document describing sampling and types of samples collected.
Reagents for CARD-FISH for dataset 805612 filename: Reagents_for_CARD-FISH.pdf (Portable Document Format (.pdf), 139.05 KB) MD5:0f8561bc90fb4c7214b3a0123327cd84 Contains information relevant to accomplishing in situ hybridization.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1851012

Deployments

None available yet

Instruments

GO-FLO Bottle

Supplied Name: Go-Flo bottle

Supplied Description:

~2L Go-Flo bottle for sampling water

Instrument Type

Generic Name: GO-FLO Bottle

Acronym: GO-FLO

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/30/

Generic Description:

GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths.

YSI Sonde 6-Series

Supplied Name: YSI datasonde

Supplied Description:

YSI datasonde to record temperature, salinity and oxygen

Instrument Type

Generic Name: YSI Sonde 6-Series

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0737/

Generic Description:

YSI 6-Series water quality sondes and sensors are instruments for environmental monitoring and long-term deployments. YSI datasondes accept multiple water quality sensors (i.e., they are multiparameter sondes). Sondes can measure temperature, conductivity, dissolved oxygen, depth, turbidity, and other water quality parameters. The 6-Series includes several models. More from YSI.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Sample_ID	Sample_ID C = cast (replicates 1 and 2) D = depth (1=2m; 2=3m; 3=4m)	unitless	sample
Sampling_Date	date of sampling	unitless	date
Depth	depth of sample	meters (m)	depth
Month	Month of sample as text	unitless	month
Temperature	Water temperature	degrees Celsius	temp
Salinity	Water salinity	parts per thousand (ppt)	sal
Dissolved_Oxygen	Dissolved Oxygen	miligrams per liter (mg/L)	O2
NH4	NH4	micromolar	NH4
NO2_NO3	NO2_NO3	micromolar	NO3_NO2
Moon_Phase_Illumination	Moon phase	percent illumination	no_bcodmo_term
Day_Length	length of day as hours:minutes converted to a decimal	decimal hours	length_day
Avg_wind	Average wind speed measured at the Coast Guard Station at Woods Hole	miles per hour (mph)	wind_speed
Max_Gust	Maximum wind gust speed measured at the Coast Guard Station at Woods Hole	miles per hour (mph)	wind_speed
Wind_Direction	Direction of wind as compass direction converted to degrees	degrees	wind_dir
Tide	tide level at sampling (low=1;slack=2;high=3)	unitless	tide
Tide_text	Textual representation of tide level	unitless	tide
Vol_Filtered_mL	amount of water filteres	milliliter (mL)	vol_filt
Infection_Count	number of cells exhibiting infection by CARD-FISH	count	count
No_of_grids_counted	number of grids counted	count	count
No_of_cells	total number of cells counted	count	count
Total_euk_count	# of cells divided by # of grids counted then multiplied by microscope correction factor 7693	unknown	abundance
Percent_infected	Infection_Count divided by Total_euk_count	unitless (percent)	no_bcodmo_term
Cast	replicates (1 or 2)	unitless	cast
No_Spores	dinospores counted by CARD-FISH	count	no_bcodmo_term
Vol_Filtered	amount of water filtered	milliliter (mL)	vol_filt
spores_per_L	spore count multiplied by 8; multiplied by 50	count per liter (count/L)	no_bcodmo_term
lat	latitude of sampling location with positive values indicating North	decimal degrees	lat
lon	longitude of sampling location with negative values indicating West	decimal degrees	lon

Database

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Dataset: 2018 Salt Pond