Dataset: LT50 Copepod Pressure Vessel Experiment Fkt230627

Name: Copepod Pressure Vessel Experiments from samples collected from the East Pacific Rise during ROV SuBastian deployments and R/V Falkor (too) cruise FKt230627 in June-July 2023
Published: 2024-03-20
Keywords: hydrothermal vents, copepod, thermal tolerance, biota, oceans

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Spatial Extent: N:9.98494 E:-104.29138 S:9.83846 W:-104.294

Near 9 North on EPR at vent sites Tica, Biovent, and Bio9

Temporal Extent: 2023-07-07 - 2023-07-21

Project:

The Underworld of Hydrothermal Vents (Vent Underworld)

Principal Investigator:

Sabine Gollner (Royal Netherlands Institute for Sea Research, NIOZ)

Co-Principal Investigator:

Monika Bright (University of Vienna)

Student:

Alessandro Messora (Royal Netherlands Institute for Sea Research, NIOZ)

BCO-DMO Data Manager:

Dana Stuart Gerlach (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-03-20

Restricted:

Validated:

Current State:

Preliminary and in progress

Copepod Pressure Vessel Experiments from samples collected from the East Pacific Rise during ROV SuBastian deployments and R/V Falkor (too) cruise FKt230627 in June-July 2023

Abstract:

These data show results from LT50 experiments from deep-sea hydrothermal vent copepods (family Dirivultidae) living associated with pompeii worm and mussel/tubeworm habitats at the 9°50’ North East Pacific Rise. Specimens were collected with a ROV at the vent sites Tica, Biovent and Bio9 in ~2500 meters water depth during expedition FKt230627 in July 2023. Experiments were performed onboard of the research vessel. Copepods were transferred to glass vials, glass vials placed into pressure vessels and pressurized. Diverse exposure times, temperatures, pressures, and oxic/anoxic conditions were selected to explore the tolerance of deep-sea hydrothermal vent copepods in relation to habitat, temperature, oxygen concentration and pressure. The data were collected by Alessandro Messora, under the supervision of Dr. Sabine Gollner (Royal Netherlands Institute for Sea Research (NIOZ), NL) and Prof. Monika Bright (University of Vienna, AUT).

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Methods & Sampling

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Funding Sources

Funding Source	Award Number
Schmidt Ocean Institute	FKt230627
Netherlands Organization of Scientific Research	OCENW.M.22.080 (Subsurface life at deep-sea hydrothermal vents)

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
FKt230629		2023-06-29	R/V Falkor (too)	Monika Bright (Chief Scientist)	data info

Instruments

ARGOLAB Water Bath 12 Liters

Supplied Name: ARGOLAB WB 12 Lt

Supplied Description:

Glass vials were placed into pressure vessels and placed in a water bath (ARGOLAB WB 12 Lt) to keep a constant temperature.

Instrument Type

Generic Name: ARGOLAB Water Bath 12 Liters

Acronym: ARGOLAB WB 12 Lt

Generic Description:

ARGOLab waterbath without recirculation pump.

Working range: +5°C above ambient temperature to 100°C.
Tank capacity: 12 liters.
Electronic temperature regulator with integrated PID control
Visual and acoustic alarms that can be silenced by the operator.
Safety class 2 according to DIN 12880.
Temperature stability at 37 °C: ± 0.1 °C.
Temperature accuracy: ± 0.2 °C.
Digital timer with programming range 1 min - 99 h and 59 min, and continuous operation.
Rack capacity: up to 4 modules.
Tank dimensions (W x H x D): 300 x 240 x 200 mm
Weight: 12 Kg.

Power supply: 220 ± 10% V / 50-60 Hz, Power: 900 W.

Microscope - Optical

Supplied Name: Leica EZ4 W Compound Microscope

Supplied Description:

At the end of each incubation the content of each vial was examined under a stereomicroscope to record the number of living/dead copepods.

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Multi-chamber Suction Sampler

Supplied Name: ROV SuBastian Multi-Chamber Suction Sampler

Supplied Description:

Specimens were collected with ROV SuBastian suction sampler at the vent sites.

Instrument Type

Generic Name: Multi-chamber Suction Sampler

Acronym: 'slurp' sampler

Generic Description:

An underwater device that collects animals and other samples under gentle suction and deposits them into a collection chamber. Also known as an oceanographic 'slurp' sampler. The primary components are an electrical or hydraulic pump, outlet hose connected to collection chamber, and inlet hose.

Oxygen Sensor

Supplied Name: Fibox 4 stand-alone fiber optic oxygen meter

Supplied Description:

Oxygen levels were measured with a Fibox 4 fiber optic oxygen meter to monitor whether the samples were oxic or anoxic.

Instrument Type

Generic Name: Oxygen Sensor

Acronym: Dissolved Oxygen Sensor

Generic Description:

An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed

Oxygen Sensor Spot

Supplied Name: Self-adhesive Oxygen Sensor Spot SP-PSt3-SA

Supplied Description:

(*** Need informaiton from the submitter to be put in here about how they were used...***)

Instrument Type

Generic Name: Oxygen Sensor Spot

Acronym: Oxygen Sensor Patch

Generic Description:

Oxygen sensor spots allow oxygen measurements in closed sample containers with contactless read-out through a transparent window (glass, acryl glass) with special adapters and optical fibers. These patches often have a self-adhesive option.

Pressure Vessel

Supplied Name: customized pressure vessel

Supplied Description:

Pressure vessels are custom items (see Klose et al. 2015; www.pnas.org/cgi/doi/10.1073/pnas.1501160112)

Instrument Type

Generic Name: Pressure Vessel

Acronym: pressurized container

Generic Description:

A pressure vessel is a container designed to hold gases or liquids at a pressure substantially different from the ambient pressure. Construction methods and materials may be chosen to suit the pressure application, and will depend on the size of the vessel, the contents, working pressure, mass constraints, and the number of items required. Examples include glassware, autoclaves, compressed gas cylinders, compressors (including refrigeration), vacuum chambers and custom designed laboratory vessels.

Pump

Supplied Name: Waters 515 HPLC Pump

Supplied Description:

Glass vials were placed into pressure vessels and pressurized using a Waters 515 HPLC Pump up to 200 bar (3000 psi).

Instrument Type

Generic Name: Pump

Generic Description:

A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps

ROV SuBastian

Supplied Description:

Specimens were collected with ROV SuBastian at the vent sites Tica, Biovent and Bio9 using suction sampler and/or manipulator grabs.

Instrument Type

Generic Name: ROV SuBastian

Generic Description:

ROV SuBastian is operated from the research vessel Falkor and the R/V Falkor(too). The ROV is outfitted with a suite of sensors and scientific equipment to support scientific data and sample collection, as well as interactive research, experimentation, and technology development. More information available at https://schmidtocean.org/technology/robotic-platforms/4500-m-remotely-operated-vehicle-rov/

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Database

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Dataset: LT50 Copepod Pressure Vessel Experiment Fkt230627