Instruments

The ECO BB measures scattering at 117 degrees, the angle determined as a minimum convergence point for variations in the volume scattering function (VSF) induced by suspended materials and water itself. As a result, the signal measured by this meter is less determined by the type and size of the materials in the water and is more directly correlated to the concentration of the materials. Conversely, the meter provides unparalleled accuracy for any single-angle measurement in determining the optical backscattering coefficient: an important parameter for remote sensing and in many in-water bio-optical applications.

An electronic jig machine is used to mechanically jig a fish hook or lure with a bait casting reel without using the fishing rod to jig the lure. Normally to jig a fish hook or lure one must move the fishing rod either vertically, horizontally, or jerk the fishing line by hand. The jigging action of this bait cast reel (how rapid and how long in distance the jig will travel) will determine the desired intensity and resonance of the rattle used in the lure to attract or snag the fish. With very simple controls, the equipment functions automatically since it is programmed to suit the actual fishing area, the fishing method and the type of fish.

A high-resolution (HR) inductively coupled plasma (ICP) mass spectrometer (MS) composed of a dual mode secondary electron multiplier (SEM) and a Faraday detector. The ELEMENT XR instrument has a dynamic range of 5 x 10^7 to 1 x 10^12 counts per second (cps), and allows simultaneous measurement of elements at concentrations over 1000 ug/g.

This radiometer measures sun and sky irradiance in the range of wavelengths 0.285 to 2.8 microns, including most of the solar spectrum. The PSP is intended to weight the energy flux in all wavelengths equally. It is a "hemispheric receiver" intended to approximate the
cosine response for oblique rays. The Eppley Precision Spectral Pyranometer (PSP) is primarily used where high accuracy is required or where it is used to calibrate other pyranometers. The PSP outputs a low level voltage ranging from 0 to a maximum of about 12mV depending on sensor calibration and radiation level. An instruction manual provided by Eppley contains the sensor calibration constant and serial number. The Precision Spectral Pyranometer is a World Meteorological Organization First Class Radiometer and comes with a calibration certificate traceable to the World Radiation Reference and a temperature compensation curve. More information is available from Eppley Labs.

The MBARI Environmental Sample Processor—the ESP—provides on-site (in situ) collection and analysis of water samples from the subsurface ocean. The instrument is an electromechanical/fluidic system designed to collect discrete water samples, concentrate microorganisms or particles, and automate application of molecular probes and qPCR which identify microorganisms and their gene products. The ESP also archives samples so that further analyses may be done after the instrument is recovered.

Environmental Sample Processor

See references below for methodology used on the ESP:
Greenfield, D.I., R. Marin III, S. Jensen, E. Massion, B. Roman, J. Feldman, C. Scholin (2006). Application of the Environmental Sample Processor (ESP) methodology for quantifying Pseudo-nitzschia australis using ribosomal RNA-targeted probes in sandwich and fluorescent in situ hybridization.  Limnology and Oceanography: Methods 4: 426-435.

Greenfield, D., R. Marin III, G.J. Doucette, C. Mikulski, S. Jensen, B. Roman, N. Alvarado, and C.A. Scholin (2008).  Field applications of the second-generation Environmental Sample Processor (ESP) for remote detection of harmful algae: 2006-2007.  Limnology and Oceanography: Methods 6: 667-679.

Marin III, R., and C. Scholin (2010). Sandwich Hybridization.  In: Microscopic and molecular methods for quantitative phytoplankton analysis (Chapter 12), edited by B. Karlson, C. Cusack, and E. Bresnan, E.. IOC Manuals and Guides, no. 55. (IOC/2010/MG/55)  Paris, UNESCO. 110 pp.

Ottesen, E.A., R. Marin III, C.M. Preston, C.R. Young, J.P. Ryan, C.A. Scholin, and E.F. DeLong (2011). Metatranscriptomic analysis of autonomously collected and preserved marine bacterioplankton. The ISME Journal, 5: 1881-1895, doi: 10.1038/ismej.2011.70.

Ottesen, E.A., C.R. Young, J.M. Eppley, J.P. Ryan, F.P. Chavez, C.A. Scholin, and E.F. DeLong (2013). Pattern and synchrony of gene expression among sympatric marine microbial populations. Proceedings of the National Academy of Sciences, 110: E488-E497, doi: 10.1073/pnas.1222099110.

Ottesen, E.A., C.M. Young, S.M. Gifford, J.M. Eppley, R. Marin III, S.C. Schuster, C.A. Scholin, and E.F. DeLong (2014). Multispecies diel transcriptional oscillations in open ocean heterotrophic bacterial assemblages. Science, 345: 207-212, 10.1126/science.1252476.

Preston, C.M., A. Harris, J.P. Ryan, B. Roman, R. Marin III, S. Jensen, C. Everlove, J. Birch, J.M. Dzenitis, D. Pargett, M. Adachi, K. Turk, J.P. Zehr, and C.A. Scholin (2011). Underwater application of quantitative PCR on an ocean mooring. PLoS ONE, 6:e22522, doi: 10.1371/journal.pone.0022522.

Robidart, J.C., C.M. Preston, R.W. Paerl, K.A. Turk, A.C. Mosier, C.A. Francis, C.A. Scholin, and J.P. Zehr (2011). Seasonal Synechococcus and Thaumarchaeal population dynamics examined with high resolution with remote in situ instrumentation. The ISME Journal, 6: 513-523, doi: 10.1038/ismej.2011.127.

Robidart, J., M.J. Church, J.P. Ryan, F. Ascani, S.T. Wilson, D. Bombar, R. Marin III, K.J. Richards, D.M. Karl, C.A. Scholin, and J.P Zehr (2014). Ecogenomic sensor reveals controls on N2-fixing microorganisms in the North Pacific Ocean. The ISME Journal, 8: 1175-1185, 10.1038/ismej.2013.244.

Saito, M.A., V.V. Bulygin, D.M. Moran, C. Taylor, and C. Scholin (2011). Examination of microbial proteome preservation techniques applicable to autonomous environmental sample collection. Frontiers in Aquatic Microbiology, 2: doi: 10.3389/fmicb.2011.00215.

Scholin, C.. (2010).  What are “ecogenomic sensors?” A review and thoughts for the future. Ocean Science 6: 51-60.

Ussler III, W., C.M. Preston, P. Tavormina, D. Pargett, S. Jensen, B. Roman, R. Marin III, S.R. Shah, P.R. Girguis, J.M. Birch, V.J. Orphan, and C. Scholin (2013). Autonomous application of quantitative PCR in the deep sea: In situ surveys of aerobic methanotrophs using the deep-sea Environmental Sample Processor. Environmental Science and Technology, 47: 9339–9346, doi: 10.1021/es4023199.

Varaljay, V.A, J. Robidart, C.M. Preston, S.M. Gifford, B. Durham, A.S. Burns, J.P. Ryan, R. Marin III, R.P. Kiene, J.P Zehr, C.A. Scholin, M. Moran. 2015. Single-taxon field measurements of bacterial gene regulation controlling DMSP fate. ISME Journal, doi:10.1038/ismej.2015.23|

An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques.

A flame ionization detector (FID) is a scientific instrument that measures the concentration of organic species in a gas stream. It is frequently used as a detector in gas chromatography. Standalone FIDs can also be used in applications such as landfill gas monitoring, fugitive emissions monitoring and internal combustion engine emissions measurement in stationary or portable instruments.

Used to catch fish.

Pound nets are passive, stationary gear used for live entrapment of fish species. The gear is composed of fiber netting. Floating pound nets use floating toggles and buoys at the surface and are held taught below the surface using anchors.


A pound net consists of: (1) a net body or crib where the entrapment takes place, (2) a least one mesh heart that helps funnel fish into the crib, and (3) a straight leader or hedging which leads fish toward the crib. Fish swimming along the shore are turned toward the crib by the hedging, guided into the heart, and into the crib where they are removed.



(Description from Maryland Dept of Natural Resources)

Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)

Imaging cytometers are automated instruments that quantify properties of single cells, one cell at a time. They combine some aspects of flow cytometry with particle imaging capabilities in an automated device to classify small particles, including phytoplankton and protozoa. They can measure a variety of properties: cell size, cell granularity, cell aspect ratio, equivalent spherical diameter (ESD) and area-based diameter (ABD) [to estimate bio-volume, which is used to estimate cell carbon biomass]. Particle images are digitally recorded and sorted into different classes according to training libraries using a support vector machine (supervised learning methods). The instruments particle-size is calibrated using different sizes of latex beads.

The FlowCam VS series are automated imaging-in-flow instruments that generate high-resolution digital images for measuring size and shape of microscopic particles. The sample introduced in the system is attracted by a peristaltic or a syringe pump into a flow cell (or flow chamber) with known dimensions, located in front of a microscope objective which is connected to a camera video. The benchtop model is ideally suited to a typical laboratory environment with applications in oceanographic research, municipal water, biopharmaceutical formulations, chemicals, oil and gas, biofuels, and many other markets. FlowCam VS is available in four models, from the imaging-only VS-I (i.e. without excitation wavelength or fluorescence emission wavelengths) to the top-of-the-line VS-IV with two channels of fluorescence measurement and scatter triggering capabilities. The instrument can measure particles between 2µm and 2mm; can analyse in vivo or fixed samples; has a flow rate between 0.005 ml/minute and 250 ml/minute (dependant upon magnification, flow cell depth, camera frame rate, efficiency desired, etc.). It can produce either 8-bit Grayscale (Monochrome Camera) or 24-bit Colour (Colour Camera) images, depending on the model.

A Folsom Plankton Splitter is used for sub-sampling of plankton and ichthyoplankton samples.

A lyophilizer, also known as freeze dryer or liofilizador, is a device that is used to freeze-dry material.

In Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, the mass-to-charge ratio (m/z) of an ion is experimentally determined by measuring the frequency at which the ion processes in a magnetic field. These frequencies, which are typically in the 100 KHz to MHz regime, can be measured with modern electronics making it possible to determine the mass of an ion to within +/- 0.000005 amu or 5 ppm.

Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.

An on-line gas preparation and introduction system for isotope ratio mass spectrometry that is designed for high precision isotope and molecular ratio determination of headspace samples, including water equilibration, carbonates and atmospheric gases. The instrument allows for the use of a dual viscous flow inlet system of repetitive measurements of sample and standard gas on a continuous flow isotope ratio mass spectrometer (CF-IRMS) system. The sample volume is the sample vial (instead of a metal bellows), and the reference gas volume is a pressurized gas tank. The instrument consists of a user programmable autosampler, a gas sampling system, a maintenance-free water removal system, a loop injection system, an isothermal gas chromatograph (GC), an active open split interface, a reference gas injection system with three reference ports, and one or two optional LN2 traps for cryofocusing. The gas sampling system includes a two port needle which adds a gentle flow of He into the sample vial, diluting and displacing sample gas. Water is removed from the sample gas through diffusion traps. The loop injector aliquots the sample gas onto the GC column, which separates the molecular species. The reference gas injection system allows accurate referencing of each sample aliquot to isotopic standards. The system can be used with several options including a carbonate reaction kit that allows injection of anhydrous phospohric acid into sample vials.

Integrated Ocean Drilling Program borehole CORK (Circulation Obviation Retrofit Kit) observatories provide long-term access to hydrothermal fluids circulating within the basaltic crust (basement), providing invaluable opportunities to study the deep biosphere. We describe the design and application parameters of the GeoMICROBE instrumented sled, an autonomous sensor and fluid sampling system. The GeoMICROBE system couples with CORK fluid delivery lines to draw large volumes of fluids from crustal aquifers to the seafloor. These fluids pass a series of in-line sensors and an in situ filtration and collection system. GeoMICROBE’s major components include a primary valve manifold system, a positive displacement primary pump, sensors (e.g., fluid flow rate, temperature, dissolved O2, electrochemistry-voltammetry analyzer), a 48-port in situ filtration and fluid collection system, computerized controller, seven 24 V-40 A batteries and wet-mateable (ODI) communications with submersibles. This constantly evolving system has been successfully connected to IODP Hole 1301A on the eastern flank of the Juan de Fuca Ridge. 

Reference: Cowen, J.P., Copson, D., Jolly, J., Hsieh, C.-C., Matsumoto, R., Glazer, B.T. et al. (2012) Advanced instrument system for real-time and time-series microbial geochemical sampling of the deep (basaltic) crustal biosphere., Deep-Sea Research I, 61: 43-56 doi:10.1016/j.dsr.2011.11.004

GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths.

Instruments that separate components in aqueous or organic solution based on molecular size generally for molecular weight determination. Gel permeation chromatography (GPC) is a type of size exclusion chromatography (SEC), that separates analytes on the basis of size.

The Global Positioning System (GPS) is a U.S. space-based radionavigation system that provides reliable positioning, navigation, and timing services to civilian users on a continuous worldwide basis. The U.S. Air Force develops, maintains, and operates the space and control segments of the NAVSTAR GPS transmitter system. Ships use a variety of receivers (e.g. Trimble and Ashtech) to interpret the GPS signal and determine accurate latitude and longitude.

The Greene Bomber is a ENDECO V-fin towed body with overall dimensions of length: 139.7 cm; width at front: 66 cm; width at rear: 142.2 cm; height: 48.26 cm. It is constructed primarily of fiberglass. Since the early 1990's it has been towed just below the sea surface with acoustic and environmental sensors to provide continuous profiles of the water column acoustic backscattering and target strengths from zooplankton with a size range of ~ 1.5 mm to 100 mm, and sea surface environmental properties (temperature, salinity, and fluorescence). It was first used with a BioSonics dual-beam acoustic system operating at 420 kHz and 1 MHz or 120 and 420 kHz. The environmental sensing system (ESS) was the ESS used on MOCNESS. In 1997 the acoustics were changed to a HTI acousitic system with 120 and 420 kHz transducers. In 2010, two additional HTI transducers (43 and 200 kHz) were added. For additional detail see:

Wiebe, P. and C. Greene. 1994. The use of high frequency acoustics in the study of zooplankton spatial and temporal patterns. Proc. NIPR Symp. Polar Biol. 7: 133-157.

Wiebe, P.H., D. Mountain, T.K. Stanton, C. Greene, G. Lough, S. Kaartvedt, J. Dawson, and N. Copley. 1996. Acoustical study of the spatial distribution of plankton on Georges Bank and the relation of volume backscattering strength to the taxonomic composition of the plankton. Deep-Sea Research II. 43: 1971-2001.

Wiebe, PH; Stanton, T K; Benfield, M C; Mountain, D G; Greene, CH. 1997. High-frequency acoustic volume backscattering in the Georges Bank coastal region and its interpretation using scattering models. IEEE Journal of Oceanic Engineering22(3): 445-464.

Compass with a motorized gyroscope that tracks true north (heading).

A hand net (also called a scoop net or dip net) is a net or mesh basket held open by a hoop. They are used for scooping fish near the surface of the water.

Handline fishing, or handlining, is a fishing technique where a single fishing line is held in the hands. A handline is a relatively large diameter line that can be pulled by hand, and it has a jig attached at the end. Handlines are frequently used for catching fish or squid that are schooling near the surface, thus a long haul by hand is not necessary.

The Hensen Net was invented by Christian Andreas Victor Hensen (February 10, 1835 - April 5, 1924) who was a German researcher considered by many to be the 'father' of Biological Oceanography. Hensen is credited with first using the term 'plankton', and the Hensen Net is one of several sampling devices he invented to help with sample collection. The Hensen Net has a 'reducing cone' forward of the net mouth, a simple closure system and a cod end collection device.

References:
Hensen, V., 1887. Uber die Bestimmung des Planktons oder des im Meere treibenden Materials an Pflanzen und Thieren. Berichte der Kommssion wissenschaftlichen Untersuchung der deutschen Meere in Kiel 5, pp. 1-107.

Hensen, V., 1895. Methodik der untersuchungen. Ergebnisse der Plankton-Expedition der Humbolt-Stiftung, Lipsius and Tischer, Kiel.

Membrane desolvation module that reduces solvent derived polyatomic interferences in the ICPMS mass spectrum. Both aqueous and organic solutions can be desolvated.

The HOBO U20L is designed for monitoring changing water levels in a variety of applications including tidal areas, streams, lakes, wetlands, and groundwater. It outputs pressure, water level, and temperature data. The instrument can record samples, sensor measurements at each logging interval, and events data, occurrences such as a bad battery or host connected. The samples are recorded as absolute pressure values, which are later converted to water level readings using software. Absolute pressure is atmospheric pressure plus water head. The deployment of an additional HOBO U20L at the surface can be used to compensate for barometric pressure changes. Each instrument is individually calibrated. They require a coupler and optic base station or HOBO waterproof shuttle to connect to a computer. The instrument is operated with a 3.6 V lithium battery.

This series contains 3 models, U20L-01, U20L-02, and U20L-04, with different operation ranges, calibrated ranges, and burst pressures. The pressure sensor is temperature compensated between 0 and 40 degrees Celsius (C), and calibrated between 69 and a maximum of 400 kPa (depending on the model). Its accuracy is within 0.3 % of the full scale for absolute pressure, and 0.1 % FS for water level readings. The temperature sensor operates between -20 and 50 degrees C, with an accuracy of 0.44 deg C, and a resolution of 0.1 deg C. The drift is 0.1 deg C per year.

The Hydrothermal Organic Geochemistry (HOG) sampler is designed to collect large volume (2–9 L) fluid samples with minimal introduction of organic or microbial contamination, and to be powered and deployed in real time from a submersible. Additional design constraints include utilizing materials appropriate for sampling fluids with elevated temperatures, fitting the sampler into the space available on the submersible, and minimizing the time needed to remove samples and prepare the sampler for re-deployment between dives. 

A type of fishing methodology sometimes used to collect fish census data. It is a hook at the end of a line.

"High-Performance Computing" (HPC) refers to a class of evolving technologies that provide leading-edge computational capabilities, including scalable high-performance computers, high-end graphic systems, and high-speed networks. HPC may be used for molecular modeling, genome analysis, and image processing, among others.

Nereus is an efficient, multi-purpose “hybrid” vehicle that can explore and operate in the crushing pressures of the greatest ocean depths. An unmanned vehicle, Nereus operates in two complementary modes. It can swim freely as an autonomous underwater vehicle (AUV) to survey large areas of the depths, map the seafloor, and give scientists a broad overview. When Nereus locates something interesting, the vehicle’s support team can bring the vehicle back on board the ship and transforms it into a remotely operated vehicle (ROV) tethered to the ship via a micro-thin, fiber-optic cable. Through this tether, Nereus can transmit high-quality, real-time video images and receive commands from skilled pilots on the ship to collect samples or conduct experiments with a manipulator arm.

Technical specifications:

• Weight on land: 2,800 kg
• Payload capacity: 25 kg
• Maximum speed: 3 knots
• Batteries: rechargable lithium ion, 15 kilowatt hours in two pressure housings
• Thrusters: 2 fore and aft, 2 vertical, 1 lateral (ROV mode) 2 fore and aft, 1 vertical (AUV mode)
• Lights: variable output LED array, strobes
• Manipulator arm: Kraft TeleRobotics 7-function hydraulic manipulator
• Sonar: scanning sonar, forward look and profile, 675 KHz
• Sensors: magnetometer, CTD (to measure conductivity, temperature, and depth)

Nereus supports a variety of science operations: Push coring, measuring heat flow, geotechnical and geochemical sensing, rock sampling and drilling, biological sampling, water sampling, high resolution acoustic bathymetry, and optical still and video imagery.

More information is available from the operator site at URL.

Multi-parameter probes that can measure from 12 (MS5) to 16 (DS5 and DS5X) parameters simultaneously. Measurements include temperature, depth, conductivity, salinity, specific conductance, TDS, pH, ORP, dissolved oxygen, turbidity, chlorophyll a, blue-green algae, Rhodamine WT, ammonium, nitrate, chloride, PAR and total dissolved gases. These probes can be deployed at depths up to 200 m and can be used in continuous monitoring programs.

The Satlantic Hyperspectral Surface Acquisition System (HyperSAS) radiometer is an above-water optical sensing system designed to provide continuous ocean color measurements over the spectral range 350-800 nm. The HyperSAS can be mounted on ships and fixed platforms, or on aircrafts for remote sensing surveys. The standard configuration of the system includes one irradiance sensor to measure downwelling irradiance, and two hyperspectral radiance sensors to capture the sea surface signal. The irradiance sensor response is proportional to the cosine of the angle of incidence of the incoming radiation, while each radiance sensor has a 3 deg field of view (FOV). The orientation precision, geo-referencing and time-stamp accuracy may be improved by mounting an optional GPS unit with Satlantic tilt and heading sensor. Moreover, a radiation pyrometer may also be added to measure land or sea surface temperature.

An ice corer is used to drill into deep ice and remove long cylinders of ice from which information about the past and present can be inferred. Polar ice cores contain a record of the past atmosphere - temperature, precipitation, gas content, chemical composition, and other properties. This can reveal a broad spectrum of information on past environmental, and particularly climatic, changes. They can also be used to study bacteria and chlorophyll production in the waters from which the ice core was extracted.

An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer.

The Imaging FlowCytobot (IFCB) is an in-situ automated submersible imaging flow cytometer that generates images of particles in-flow taken from the aquatic environment. https://mclanelabs.com/imaging-flowcytobot/

A trawl with a pentagonal mouth opening and a dihedral depressor vane as part of the mouth opening. IKMTs come in various dimensions (refer to individual dataset documentation).

The original IKMTs were 10 foot (304 cm) and 15 foot (457 cm) at the mouth. The 10 foot IKMT net was 31 feet (9.45 m) in length (Wiebe and Benfield 2003).

Submersible heating element for water tanks and aquaria.

The tracer injection sled is an integrated instrument package that is deployed from a vessel and lowered to the target potential density surface and then towed along that surface at 0.5 m/s while the tracer is injected. The sled is neutrally buoyant and is towed at the end of a 2-meter tether attached to the end of the CTD cable, which removes much of the ship motion. To follow lower frequency displacements of the target surface, the winch is controlled automatically with feedback from a SeaBird 911plus CTD system (Ledwell et al., 1998).The CTD has dual pumped C/T sensors mounted at the front of the sled to sample water not perturbed by the thermal wake of the sled. A WET Labs fluorometer is also present along with mounted injection orifices, pumps, batteries, fluid reservoirs, and control electronics for the injection.

Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

The Satlantic ISUS nitrate sensor is an in-situ UV absorption sensor which calculates nitrate concentration from the seawater spectrum. The ISUS V2 has a 1cm path length, a 200-400 nm wavelength range., and is depth rated to 1000 m. Satlantic's ISUS V3 nitrate sensor uses advanced UV absorption technology to measure nitrate concentration in real-time.

A JelNet is a plankton net system specifically designed to quantify fragile zooplankton ("jellies"). The net is 1.5m^2 at its opening and tapers to a shallow 50 cm deep flat bottom which has a collection "sock" to which a standard MOCNESS cod collar and end bucket were attached. Mesh size of the net is 5mm (3/16 inch). The square net frame is 16mm (5/8 inch) stainless steel and is bridled from each of the corners to a common point above by a 5mm (3/16 inch) stainless steel wire with thimbles spliced into the wire ends. A 45-kg lead ball is attached to a 13mm ( 1/2 inch) oblong stainless ring when the net is fished.

The Knudsen 320 B/R deepwater echosounder is a digital data logging system used to measure water depth (e.g. depth of the seafloor). The system is configured to work with different frequency transducers. For example, the Edo 323 B is a 12 kHz High Frequency (HF) transducer or it can be configured to work with an array of 3.5 kHz Low Frequency (LF) transducers mounted in the hull of a vessel.

The Large Aggregate Profiling System (LAPS) is a camera system developed to characterize millimeter size particle distributions in the water column. Camera systems are integrated with a CTD and transmissometer and therefore have the advantage of simultaneously collecting data on the distribution of suspended particles and aggregates along with the physical structure of the water column (Honjo et al., 1984; Asper, 1987; Gardner et al., 1988).

"The LI-1000 DataLogger is a 10 channel datalogger that functions both as a data logging device and a multichannel, autoranging meter. The electronics of the LI-1000 have been optimized for highly accurate measurement of LI-COR radiation sensors which have a current signal" (from LI-COR Datalogging Instruction Manual, p 1). LI-COR began manufacturing these instruments in 1985 and discontinued in 1998. Serial Numbers for this model have the prefix of LDL-XXXX. (www.licor.com)

The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973.

These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device.

The LI-192 differs from the LI-193 primarily in sensitivity and angular response.
193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis.
192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use.
(www.licor.com)

The LI-200R Pyranometer measures global solar radiation (the combination of direct and diffuse solar radiation) in the 400 to 1100 nanometer (nm) wavelength range. Measurement units are in watts per square meter (W m-2). It is used for applications in agricultural, meteorological, solar energy, and environmental research. The LI-200R measures global solar radiation with an unfiltered silicon photodiode. The crown of the sensor rapidly sheds water, and also physically blocks light from outside the hemisphere of sensitivity, providing a precise cosine response. Its sensitivity is typically 75 uA per 1000 W m-2. It has a maximum deviation of 1% up to 3000 W m-2. Its stability is < +/- 2% change over a 1-year period. It has an operating temperature range of -40 to +65 degrees Celsius.

The LI-7000 CO2/H2O Gas Analyzer is a high performance, dual cell, differential gas analyzer. It was designed to expand on the capabilities of the LI-6262 CO2/ H2O Gas Analyzer. A dichroic beam splitter at the end of the optical path provides radiation to two separate detectors, one filtered to detect radiation absorption of CO2 and the other to detect absorption by H2O. The two separate detectors measure infrared absorption by CO2 and H2O in the same gas stream. The LI-7000 CO2/ H2O Gas Analyzer is a differential analyzer, in which a known concentration (which can be zero) gas is put in the reference cell, and an unknown gas is put in the sample cell.