Hydrographic profiles and water samples were collected with a standard CTD-rosette system with Niskin bottles.\u00a0 Nutrient samples were filtered through Millipore HA filters, placed immediately in a sea water-ice bath for 5\u201310 min, and frozen at \u221218\u00b0C.\u00a0 Concentrations of NO3+NO2, NH4, Si(OH)4 and PO4 were measured with a Bran Luebbe AA3 AutoAnalyzer using standard techniques.
\nA. fundyense cells were enumerated from water samples using an oligonucleotide probe and methods described in Anderson et al. (2005). Both A. tamarense and A. fundyense occur in the Gulf of Maine, and these are considered to be varieties of the same species. Available molecular probes cannot distinguish between them, and only detailed analysis of the thecal plates on individual cells can provide this resolution\u2014which is not practical for large numbers of field samples.\u00a0 Accordingly, for the purpose of this study, the name A. fundyense is used to refer to both forms.
\nAnderson, D. M., D. M. Kulis, B. A. Keafer, K. E. Gribble, R. Marin, and C. A. Scholin. 2005. Identification and enumeration of Alexandrium spp. from the Gulf of Maine using molecular probes. Deep-Sea Research II 52: 2467-2490.
Multi year bottle data 2003-2010
\nNote: Dataset updated with 2008/EN448 Version 3 data srg/21Mar2013
These data include version 2 data as submitted.
\nVersion 2 NOTES: Version 2 means that Data set has been updated in part of the new data added: (1) 2010 cruises; (2) Previously unavailable Whole cell counts from the previous cruises; (3) Previously unavailable data for Underway stations (mostly date, location).
Data files newly created or updated in this version are indicated with \"_v2\" in file names.
\n(BCO-DMO Note: for original files as contributed)
Date of creation: 5/12/2011.
\nMatlab code used for data merging: GM_read_alex_nuts_BTL_v2.m
No data reported for cruises: OC440 (2007) or EN456 (2008).
\nData not sampled or lost are indicated with NaN.
\nData not available at the time but supposed to arrive are indicated with the \"waiting\" flag=-9.99.
Funding:
\nThe cruises from 2003-2004 were supported by NOAA grant NA160P2785 (MERHAB). The cruises from 2005-2010 were jointly funded: NSF grant OCE-0430724, NIEHS grant 1P50-ES01274201 (Woods Hole Center for Oceans and Human Health) and NOAA grant NA06NOS4780245 (GOMTOX).
Hydrographic profiles and water samples were collected with a standard CTD-rosette system with Niskin bottles.\u00a0 Nutrient samples were filtered through Millipore HA filters, placed immediately in a sea water-ice bath for 5\u201310 min, and frozen at \u221218\u00b0C.\u00a0 Concentrations of NO3+NO2, NH4, Si(OH)4 and PO4 were measured with a Bran Luebbe AA3 AutoAnalyzer using standard techniques.
\nA. fundyense cells were enumerated from water samples using an oligonucleotide probe and methods described in Anderson et al. (2005). Both A. tamarense and A. fundyense occur in the Gulf of Maine, and these are considered to be varieties of the same species. Available molecular probes cannot distinguish between them, and only detailed analysis of the thecal plates on individual cells can provide this resolution\u2014which is not practical for large numbers of field samples.\u00a0 Accordingly, for the purpose of this study, the name A. fundyense is used to refer to both forms.
\nAnderson, D. M., D. M. Kulis, B. A. Keafer, K. E. Gribble, R. Marin, and C. A. Scholin. 2005. Identification and enumeration of Alexandrium spp. from the Gulf of Maine using molecular probes. Deep-Sea Research II 52: 2467-2490.
\n\u00a0BCO-DMO Processing/Edits
\n- Generated from data in original file \"GOM_GB_nuts_TS_Alex_v2.tar.gz\" contributed by Olga Kosnyrev
\n- Awk routine \"GoMEbottle2csv.awk\" generated to convert bottle \".txt\" files to BCO-DMO .csv files
\n- Updated with version 3 data for 2008/EN448 data\u00a0 21March2013, srg
\n- All bottle data reprocessed to correct NH4 data 21March2013, srg