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2010 and 2011 Data Acquisition:
\nSampling from small boats occurred along transects at the Moorea Coral Reef LTER sites during September 2010 and September 2011. Benthic producers were collected from water depths of 1.0 to 1.5 meters at back and fringe reef locations. Bacterioplankton abundance was determined using flow cytometry (Nelson et al. 2011). DOC was measured by a high-temperature TOC analyzer according to methods of Carlson et al. (2010). To assess DOC release by primary producers, each benthic species was incubated using the method of Herndl and Velimirov (Haas et al. 2011). To determine if DOC released by primary producers affects mirobial populations, ~48 hour dark dilution culture incubations were performed to measure changes in concentration of DO, DOC, and bacterioplankton over time (Haas et el. 2011).
For more details on methodology, quality assurance and control procedures, and precision and accuracy of methods used, see the following references (full citations are below):
\nBacterioplankton flow cytometry methods: Nelson et al. 2011.
Experimental designs, dissolved oxygen, pH, temperature, and specimen surface area methods: Haas et al. 2011,\u00a0Herndl & Velimirov B. 1986.
\nDissolved organic carbon method: Carlson et al. 2010.
\nChlorophyll method: Smith et al. 1981.
\nDissolved inorganic nutrients, particulate organic nutrients, and isotope ratios methodological references, calibrations, precision, and accuracy are detailed at the UCSB MSI Analytical Lab Website.
This dataset contains experimental and survey biogeochemical and microbial data from samples collected at the Moorea Coral Reef LTER site (French Polynesia). Goals of the study were to investigate how benthic primary producers directly affect seawater chemistry (DO and DOC) and to examine impacts of released DOC on reef bacterioplankton growth and respiration. Rates of photosynthesis, respiration, and DOC relsease were assessed for several benthic reef organisms (Haas et al. 2011).
BCO-DMO made the following modifications: changed parameter names to conform with BCO-DMO conventions; replaced blanks and 'NA' with 'nd' to indicate no data; calculated month_gmt, day_gmt, and time_gmt from the local dates and times provided (local to French Polynesia (Papeete, Tahiti; UTC/GMT -10 hours; Time Zone Code TAHT)); rounded the following to 4 decimal places (originally submitted in Excel file with 11 decimal places): POC, PON, d13C, d15N, algal_area_O2, algal_area_C; replaced '<' with 'lt_' (less than) and '>' with 'gt_' (greater than) in depth_bottom column; removed parameters defined as 'internal reference code'.