In 2010, various boats, including the following, were used to sample from the research station: Gump Safe boat, boats 609, 509, and 389.
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2011 Experiments (codes in parenthesese correspond to 'project_code' column):
\nBenthic Tent Incubations (TentI - TentV) - 48h Duration; Continuous: Oxygen, pH, Temp, Salinity; Daily: DOC, FCM, DNA, start-end POM.
\nNiskin in situ incubations (NBE) - 24h Duration; Start-End DOC, FCM, DNA, DO...
\nRelease-Remineralization (RR) - 24h Exudation, 48h Remineralization (with and without Sand, Light and Dark): DOC, DO, FCM, DNA.
\nCoral Damage (Sprout Soup) - 24h Exudation, 7 day incubation (daily water change + exudate spike).
\nBig Pillow Experiments (BPE) - 24h Exudation, 48h incubation (metagenomic filtration at 48h, maintain for later DOC timepoints and FCM.
\nFlow and Flux Surveys (FF)
\nPorewater Sampling (PW)
\nSandBag (SB) - Sand, Autoclaved Sand, incubated in offshore water in ~5L bags (unfiltered).
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2011 Methodology:
\nFCM - 1.5ml sample, 90 uL PFA (8% or 32% stocks).
\nDOC - collected through 47mm combusted GF/F into acid-leached 60ml HDPE or combusted EPA.
\nnutrients - collected through 47mm combusted GF/F into triple-rinsed HDPE scint vials.
\nDNA - 500+ mL whole water collected onto Sterivex.
\nOxygen (NBE T0) - After sampling DOC, FCM, additional offsh. water to fill, optode measure, close with spill.
\nOxygen (NBE TF) - Before other samples, open niskin to laminar flow into BOD bottle, overfill 2X, DO meas.
\nPOC (NBE) - 500-1000 mL collected through 25mm combusted GF/F.
\nPOC (FF) - 4L collected through 25mm combusted GF/F.
\nChlorohpyll (FF) - 1L collected through 25mm combusted GF/F.
\nFluorescein - 60ml initial line flush, then 10ml syringe sampled emptied 15ml falcon frozen -20.
BCO-DMO made the following modifications:
\n- changed parameter names to conform to BCO-DMO conventions;
\n- replaced blanks and 'NA' with 'nd' to indicate no data;
\n- calculated date/time in GMT format from the local dates and times provided (local to French Polynesia (Papeete, Tahiti; UTC/GMT -10 hours; Time Zone Code TAHT));
\n- columns originally named 'Samples Collected' and 'Comments' were merged into a single comment field;
\n- 'et al.' used in si column when 3 or more people were listed.