At sea, animals were captured for physiological experiments using a 1 m diameter, 150 \u00b5m (in 2011) or 335 \u00b5m (in 2012) mesh Reeve net trawl, or a 1 m2 MOCNESS tow with 150 \u00b5m mesh nets. Pteropods were placed in filtered seawater at densities of < 30 individuals liter-1 and acclimated for at least 8 hours at 20\u00b0 C, 15\u00b0C or 10\u00b0C in temperature controlled waterbaths. After acclimation, individuals that were in good condition were put into glass syringe respiration chambers with a known volume of 0.2 micron filtered seawater for at least four hours.
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In 2012 on cruise NH1208, an experiment\u00a0 was conducted to determine whether antibiotics changed the metabolic rate of the pteropods. In this experiment water was treated with 25mg each of Streptomycin and Ampicillin liter-1 or without antibiotics.
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Unless otherwise explicitly stated, the water contained 25mg each of Streptomycin and Ampicillin liter-1, to prevent bacterial growth, and was bubbled with certified gas mixes to achieve normal air saturated (21% O2, 380 ppm CO2), high CO2 (21% O2, 800 ppm CO2), low O2 (10% O2, 380 ppm CO2) or low O2 high CO2 (10% O2, 800 ppm CO2) conditions. Bubbling of 10% O2 achieved a mean initial O2 concentration of 10-13% in low O2 treatments.
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During all experiments, we simultaneously ran a control syringe (without an animal)\u00a0 to monitor background respiration of microbes. At the conclusion of the experiments, we measured the O2 level by withdrawing a sample of water from the chamber using a\u00a0 500 \u00b5L airtight Hamilton syringe and injected past a Clarke-type O2 electrode (#1302) and meter (#782) in a water-jacketed injection port (#MC100, Strathkelvin Instruments, North Lanarkshire, United Kingdom; Marsh and Manahan, 1999). Our resulting O2consumption rates are reported in \u00b5moles g-1 h-1 (wet mass). Notes on carbonate chemistry and net profiles are in the cruise report.
Individuals in normoxic treatments that consumed more than 70% of the oxygen in the chamber were excluded from further analyses. Oxygen consumption was calculated by subtracting experimental O2 from control O2.
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BCO-DMO Processing:
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version 2 [2016-07-26] replaced version 1 [2012-11-06]: NH1208 longitude values were corrected to negative degrees.
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- added conventional header with dataset name, PI name, version date, reference information
\n- renamed parameters to BCO-DMO standard
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