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See Howard et al. (2010), Rinta-Kanto et al. (2011), and Vila-Coast et al. (2010) for detailed methods, summarized below:

\n

\"In October 2006, seawater was collected from surface waters (<1 m deep) in the Gulf of Mexico off the coast of Dauphin Island, AL (lat: 30 03.041N; lon: 87 59.708W). Water was filtered through a 200-um mesh into six 20-liter polyethylene Cubitainers with minimal headspace.

\n

Three microcosms were amended with 10 um sodium nitrate (NaNO3) and 0.6 um potassium phosphate (K2HPO4) to serve as the experimental microcosms. Three microcosms were left untreated to serve as the control. The Cubitainers were maintained at 27 degrees C on a 12-hour light/dark cycle for the duration of the experiment.

\n

Chemical and activity measurements were collected from the microcosms at the beginning of the experiment (Day 0) and every day for the duration of the experiment at the same time. Bacterial production was measured by 3H-leucine incorporation into trichloroacetic acid (TCA) -insoluble material. Incubations were carried out in triplicate in the dark at in situ temperature with additions of 20 nM of 3H-leucine for 4 hours, starting immediately after water collection. One TCA-killed sample was used as a control. Samples were processed by the microcentrifugation method. Bacterial sulfur requirements were estimated through conversion of bacterial heterotrophic production assuming a bacterial C/S molar ratio of 248.\"

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Bacterial production measurements from control and experimental microcosms from the Dauphin Island Cubitainer Experiment (DICE).

\n

Experimental design, methods, and results are further described in:
\nE. C. Howard, S. Sun, C. R. Reisch, D. A. del Valle, R. P. Kiene, and M. A. Moran (2010). Changes in DMSP Demethylase Gene Assemblages in Response to an Induced Phytoplankton Bloom. Applied and Environmental Microbiology, vol. 77, p. 524. DOI: 10.1128/AEM.01457-10
\nRinta-Kanto, H. Burgmann, S. M. Gifford, S. Sun, S. Sharma, R. P. Kiene, and M. A. Moran (2011). Analysis of Sulfur-Related Gene Expression by Roseobacter Communities Using a Taxon-Specific Functional Gene Microarray. Environmental Microbiology, vol. 13, p. 453. DOI: 10.1111/j.1462-2920.2010.02350.x
\nVila-Costa, M., J. M. Rinta-Kanto, S. Sun, S. Sharma, R. Poretsky, and M. A. Moran. (2010). Transcriptomic analysis of a marine bacterial community enriched with dimethylsulfoniopropionate. ISME Journal, vol. 4, p. 1410. DOI: 10.1038/ismej.2010.62

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BCO-DMO made the following changes:
\n- Modified parameter names to conform with BCO-DMO naming conventions.
\n- Replaced blanks with 'nd' to indicate 'no data'.
\n- Added the site coordinates provided in the publications above.

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