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Methods are summarized below. Detailed methods for all data collected as part of this study can be found in the publications arising from this study (references given below).
\nOn each BATS cruise, aquasi-lagrangian sampling scheme is employed. An in situ primary productivity array is deployed from dawn to dusk. The biogeochemistry and biological parameters reported in this data were measured from Niskin bottle water samples.
\nBacterial production was measured using [3H-methyl] thymidine incorporation and converted to carbon-based bacterial production using standard equations. Bacterial abundance was determined using DAPI stained epifluorescence microscopy. Pico-autotrophs were identified as either Synechococcus and Prochlorococcus.
\nSamples for NO3/NO2, NO2 and PO4 are filtered and frozen (-20 degrees C) in HDPE bottles until analysis. Total organic carbon (TOC) and total nitrogen were determined using high temperature combustion techniques. Total phosphorus concentrations are quantified using a high temperature/persulfateoxidation technique. Particulate organic carbon (POC) and nitrogen (PON) samples were filtered on precombusted Whatman GF/F filters and frozen until analysis on an elemental analyzer. Particulate phosphorus samples were analyzed using an ash-hydrolysis method with oxidation efficiency and standard recovery checks.
\nSample QA/QC procedures followed those given in the associated manuscripts. At the point of collection, any leaking niskin bottles were noted on the master cast sheets and samples were taken from a different niskin fired at the same depth as the leaking bottle. No data are reported for leaking Niskin bottles. During sample analysis, certified standards, where available, were carefully examined to ensure that they were consistent with expectations for accuracy and precision. If no obvious error or problem was found, the data were considered OK and in the range of environmental data that this study hoped to observe.
\nSample accuracy was assessed by using certified standards, for those measurements where standards are available. Certified standards were run with each analytical run and compared to long term control charts for respective analyses. For those analyses where there are no standards (e.g., flow cytometric cell counts) data were assessed for reasonableness based upon extensive experience of the PI\u2019s.
\nDetailed information on analyses:
\nLomas, M.W., Burke, A., Lomas, D.A., Bell, D.W., Shen, C., Ammerman, J.W., Dyhrman, S.T. 2010.\u00a0 Sargasso Sea phosphorus biogeochemistry: An important role for dissolved organic phosphorus (DOP). Biogeosciences 7: 695-710. doi: 10.5194/bg-7-695-2010
\nLomas, M.W., Bates, N.R., Johnson, R.J., Knap, A.H., Steinberg, D.K., Carlson, C.A. 2013. Two decades and counting: overview of 24-years of sustained open ocean biogeochemical measurements. Deep Sea Research II doi: 10.1016/j.dsr2.2013.01.008.
References:
\nCasey, J.R., Aucan, J.P., Goldberg, S.R., and Lomas, M.W. 2013. Changes in partitioning of carbon amongst photosynthetic pico- and nano-plankton groups in the Sargasso Sea in response to changes in the North Atlantic Oscillation. Deep Sea Research II doi: 10.1016/j.dsr2.2013.02.002
Biogeochemical and biological data collected on the Trophic BATS cruises in the Sargasso Sea. Data are from 4 cruises over the span 2011-2012. The provided data are complete matrices and therefore not every sample (columns) will be taken from every Niskin fired (rows).
The provided data are complete matrices and therefore not every sample (columns) will be taken from every Niskin fired (rows). Data that were either not collected, or were associated with leaking Niskins, or were found to be in error for other reasons are denoted by 'nd'. Most of the data given in this dataset are not derived variables and are calculated using reasonably standard equations as given in the appropriate references. Where data are derived (e.g., bacterial carbon biomass) the appropriate reference is given in the parameter definition.
\nOnly nutrient analyses were close to analytical method detection limits (MDL). MDLs were estimated as 3x the standard deviation of the lowest standard used for the analysis and are ~30nM for nitrate and phosphate using a standard autoanalyzer. We used the MAGIC co-precipitation method for phosphate which lowered our MDL to ~0.5nM. Samples below the MDL are reported as the MDL.
\nBCO-DMO Processing Notes:
\n- Modified parameter names to conform with BCO-DMO naming conventions.
\n- Replaced '-9.99' with 'nd'.