[Adapted from: Randall et al. 2014 Ecology 95(7) 1981-1994]\u00a0\u00a0 \u00a0
\n\u00a0\u00a0 \u00a0To assess the prevalence of coral diseases at each location, a survey area (1-10 km^2 depending on the region\u2019s geographic features) of hard-bottom habitat was visually defined using Google Earth. The survey area was divided into 100 by 100 meter cells (using Google Earth Path 1.4.4). Within each location, twenty-five 100 by 100 meter cells were randomly selected as sites. These sites were defined as the primary sampling units. A single 10 by 10 meter quadrat was haphazardly placed within each site, for field-data collection. To maintain consistency across locations and to minimize potential effects of coral-assemblage differences, three criteria had to be met for a site to be surveyed: (1) the depth averaged between 5 and 10 meters, (2) the substrate was hard bottom, and (3) corals were present. If any one of these criteria was not met at a given site, it was rejected and the next randomly generated site was selected. In total, twenty-five, 10 by 10 meter quadrats were sampled at each location, for a total of 50 quadrats across two frequent-anomaly locations and 50 quadrats across two reference locations, for a total survey area of 10,000 m^2.\u00a0
\nAll four locations were surveyed between 2 July and 1 September 2012. At each site, divers surveyed each 100 m^2\u00a0quadrat by systematically laying ten contiguous 1 x 10 m belt transects onto the reef substrate. Each coral colony with a disease sign was identified in situ and the species and disease signs were recorded. Four disease signs were identified: (1) white sign was defined as a bright, white band or patch of recent mortality adjacent to healthy-appearing tissue (i.e., the tissue bordered a well-defined edge of exposed skeleton not yet colonized by algae or other biofouling organisms) (sensu Bythell et al. 2004) , (2) dark spot was defined as tissue with purple, brown or black lesions, forming spots of irregular shapes (sensu Goreau et al. 1998), (3) black band was defined as a black band over the coral tissue exposing white skeleton with different stages of biofouling (sensu Richardson 2004), and (4) yellow sign was defined as a yellow discoloration of tissue forming a band or blotches (sensu Santavy et al. 1999). White signs and black bands were associated with recent tissue loss; yellow signs and dark spots were usually, but not always, associated with recent tissue loss. Notably, very few yellow bands were observed that followed the classical description (Reeves 1994). Instead, most coral colonies presented a patchy, non-uniform yellowing of the tissue; therefore the condition was termed \u2018yellow sign.\u2019 Additionally, any area of recently exposed white skeleton, which was not clearly caused by predation or a competitive interaction, was recorded as a white sign, including white plagues, white bands and white pox. The white-sign diseases were not differentiated because of similar- or identical-appearing signs, unknown etiologies for several diseases, and the possibility that the diseases were caused by the same pathogens (Bythell et al. 2004, Ainsworth et al. 2007). Coral colonies were occasionally recorded with two or more signs of disease, when those signs appeared to be spatially independent.
\nDisease mapping:
\nFour 100 m^2 quadrats per location were mapped in their entirety, for a total of eight 100 m^2 quadrats per temperature-stress level. Approximately 50 digital images were captured from each video-transect file using Free Video to JPG Converter v. 5.0.58 build 324. The digital images were stitched together using Adobe Photoshop\u00a0CS5 v. 12.0, and ten 1\u2013m by 10\u2013m image mosaics were created for each 10 m by 10 m site. Each photo-mosaic was printed and, with the aid of the digital images and videos, the following data were measured and recorded for every coral colony within each site: (1) species, (2) spatial coordinates, (3) maximum diameter, (4) perpendicular diameter, (5) an estimate of percent partial mortality (0, <5, 25, 50, 75, or >95 %), and (6) \u2018health\u2019 status. Corals were identified as either healthy, or as having white signs, dark spots, black bands, yellow signs, or unknown signs of disease (as described above). Bleached or pale colonies also were recorded, and when multiple disease signs were present on an individual colony, both signs were recorded. Data from each site that was mapped are found in individual excel files in the folder called Disease mapping site data files.\u00a0
This dataset contains site information including latitude, longitude, depth, and survey date.
\nCoral disease transmission experiments were completed for dark-spot syndrome on Sidereastrea siderea\u00a0and yellow-band disease on Orbicella faveolata, as described in Randall et al. 2016.\u00a0Following experimentation, microbial communities were extracted from tissue samples to determine whether any\u00a0potential pathogen may have transmitted from healthy to exposed corals. Microbial communities on healthy corals\u00a0were compared with diseased corals to identify any potential pathogens.
\nExperimental diseased and healthy corals were sampled and their microbial communities were analyzed using 454 Illumina pyrosequencing\u00a0of the amplified 16S rRNA gene on the V1-V3\u00a0hypervariable region.
Only raw data, and data obtained from image mosaics\u00a0are provided.
\nData Management Office Notes:
\n- Commas and spaces removed. Spaces were replaced with underscores.
\n- Reformatted column names to comply with BCO-DMO standards.