Samples were collected offshore from Alligator Reef (2014) and Looe Key Reef (2015-2016). Measurements included temperature, salinity, pH, abundance of Vibrio, chlorophyll-a, DOC, TDN, DON, NH4, NO3, NO2, Orthophosphate ,SiO4, and dFe.
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Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) were determined using the filtrate of water samples that were passed through precombusted 25 mm GF/F filters and stored frozen (-20C) until analysis.\u00a0 Samples were subsequently analyzed using the High Temperature Catalytic Oxidation method on a Shimadzu TOC-Vs analyzer with nitrogen module.\u00a0 Standard curves were run twice daily using a DIW blank and five concentrations of either acid potassium phthalate solution or potassium nitrate for DOC and TDN, respectively.\u00a0 Three to five subsamples were taken from each standard and water sample and injected in sequence.\u00a0 Reagent grade glucosamine was used as a laboratory check standard and inserted throughout each run, as were Certified Reference Material Program (CRMP) deep-water standards of known DOC/TDN concentration.\u00a0
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Nutrients data went through internal lab QAQC process. BDL means below detection limit. The method detection limit (MDL) was determined using 9 samples on two different runs and correct student-T value.
Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016.
BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- nd (no data) was entered into all blank cells
\n- reduced number of significant digits of Vibrio and nutrient values to 2 digits to right of decimal and lat/lon to 5 digits.
\n- re-formatted date from m/d/yyyy to yyyy-mm-dd
\n- replaced spaces with underscores