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This data set is associated with PI Douglas Bartlett (NSF OCE-1536776) and Schmidt Ocean Institute R/V Falkor cruise FK141215. The cruise occurred December 15-21, 2014 in the Challenger Deep within the territorial waters of the Federated States of Micronesia. During this cruise the Leggo lander was deployed multiple times and drops 1 and 3 recovered seawater samples that were analyzed. Additional details can be found at: https://schmidtocean.org/cruise/expanding-mariana-trench-perspectives/ and https://scripps.ucsd.edu/labs/dbartlett/contact/challenger-deep-cruise-2014/

\n

Leggo Lander Drop 1:
\nTime (in Guam) deployed/recovered: December 16, 9:00/19:26.
\nPosition at deployment: 11\u00b0 21.9836 N 142\u00b0 25.9533 E, middle section of the Challenger Deep.
\nGreatest depth of dive: approximately ~10,900 m.
\nIn situ temperature on seafloor: 2.6\u00b0C.
\nNotes: This drop recovered seawater samples from about a meter off the seafloor.\u00a0This included a 3 L\u00a0Niskin bottle of seawater and ~ 150 mls of seawater collected in a pressure-retaining seawater sampler.\u00a0The PRS sampler held more than 81% of the in situ pressure.\u00a0

\n

PRS data:
\nThe culture independent identification of the bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1. This involved cell sorting, multiple displacement amplification, and 16S rRNA gene PCR and sequencing. More complete details are described in:

\n

Leon-Zayas, R., Novotny, M., Podell, S., Shepard, C. M., Berkenpas, E., Nikolenko, S., Pevzner, P., Lasken, R. S. and Bartlett, D. H. 2015. Single cells within the Puerto Rico Trench suggest hadal adaptation of microbial lineages. Appl. Environ. Microbiol. 81: 8265-8276. doi:10.1128/AEM.01659-15

\n

Colony Identification:
\nData from the identification of bacteria cultured from the Leggo drop 1 and 3 Niskin bottles are available as a\u00a0supplemental file\u00a0(.txt).\u00a0These identifications were performed using standard methods associated with PCR amplification of the 16S rRNA gene followed by dideoxy sequencing at Retrogen Inc.\u00a0\u00a0

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Culture-independent identification of bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1.

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The only processing of the data is that the 16S rRNA gene sequences has been examined using BLAST: https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome

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