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Sample Codes:

\n

Read count parameter names (e.g. FA3_N7_SF1) include the following three codes:

\n

MOCNESS tow
\nFA3: Night sampling
\nFA4: Day sampling

\n

Depth range:
\nN1: 1500-1000m
\nN2: 1000-700m
\nN3: 700-500m
\nN4: 500-300m
\nN5: 300-200m
\nN6: 200-150m
\nN7: 150-100m
\nN8: 100-50m
\nN9: 50m-0m

\n

Wet-sieved zooplankton size fractions
\nSF1: 0.2-0.5 mm
\nSF2: 0.5-1.0 mm
\nSF3: 1.0-2.0 mm

\n

Data parameters with the prefix (NCBI_) are from the National Center for Biotechnology Information (NCBI, https://www.ncbi.nlm.nih.gov/)

\n

Data parameters with the prefix (silva_) are from the Silva database (https://www.arb-silva.de/).

","@type":"rdf:HTML"}],"http://ocean-data.org/schema/hasBriefDescription":[{"@value":"Operational taxonomic unit (OTU) tables and fasta files for representative sequences from each OTU","@language":"en-US"}],"http://purl.org/dc/terms/description":[{"@value":"

This data submission consists of metabarcoding data for the zooplankton community in the epipelagic, mesopelagic and upper bathypelagic zones (0-1500m) of the North Pacific Subtropical Gyre. The goal of this study was to assess the hidden diversity present in zooplankton assemblages in midwaters, and detect vertical gradients in species richness, depth distributions, and community composition of the full zooplankton assemblage. Samples were collected in June 2014 from Station ALOHA (22.75, -158) using a 1-meter square\u00a0Multiple Opening and Closing Nets and Environmental Sampling System (MOCNESS, 200um mesh), on R/V Falkor cruise FK140613. Next generation sequence data (Illumina MiSeq, V3 chemistry, 300-bp paired-end) of the zooplankton assemblage derive from amplicons of the V1-V2 region of 18S rRNA (primers described in Fonseca et al. 2010). The data includes sequences and read count abundance information for molecular OTUs from both holoplanktonic and meroplanktonic taxa.\u00a0\u00a0All results derive from analyses in\u00a0mothur\u00a0v1.36.1 (Schloss et al. 2009, Kozich et al. 2013).

\n

Tables from four analyses are included in this submission:

\n

1) 97_OTUtable:\u00a0read counts for each OTU (clustered at 97% similarity) across 54 samples (depth, size fractionated), with NCBI BLAST results for the top representative sequence from each OTU.\u00a0

\n

2) 97_OTU_subsampled: read counts for each OTU (clustered at 97% similarity), subsampled for even sequencing coverage across 54 samples (depth, size fractionated, with NCBI BLAST results for the top representative sequence from each OTU.

\n

3) 99_OTU: read counts for each OTU (clustered at 99% similarity) across 54 samples (depth, size fractionated), with NCBI BLAST results for the top representative sequence from each OTU.\u00a0

\n

4) 99_OTU_subsampled: read counts for each OTU (clustered at 99% similarity), subsampled for even sequencing coverage across 54 samples (depth, size fractionated), with NCBI BLAST results for the top representative sequence from each OTU.

\n

Related dataset containing NCBI accession numbers for sequence data:
\nMetabarcoding zooplankton at station ALOHA: NCBI SRA accession numbers\u00a0www.bco-dmo.org/dataset/700961

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BCO-DMO processing notes:
\n* commas in the data were replaced with semicolons to support export as\u00a0csv\u00a0format.
\n* fasta files added to dataset

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