DIC:\u00a0Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m.\u00a0 DIC was measured on mercuric chloride poisoned samples by acidification and subsequent quantification of released CO2\u00a0using a CO2\u00a0detector (Li-Cor 7000). DIC samples were collected following recommended procedures {Dickson et al., 2007} and measurements were calibrated against Certified Reference Materials provided by Dr. A. G. Dickson at Scripps Institution of Oceanography (SIO), University of California, San Diego (UCSD). \u00a0\u00a0
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pH:\u00a0Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m.\u00a0 pH was measured spectrophotometrically {Clayton and Byrne, 1993} in triplicate at standard temperature (25\u00b0C) immediately following collection. pH samples were collected following recommended procedures {Dickson et al., 2007}.
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Secchi Depth: Secchi depth was measured in duplicate using a 20 cm disk with four alternating white and black quadrants (Cole Parmer #EW-05492-00) by lowering the disk until no longer visible and recording the depth.
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Salinity: Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m.\u00a0 Salinity was measured using a calibrated handheld digital refractometer (Atago PAL-06S), using a refractometer (Vista A366ATC), or using a Guideline Portasal 8410A all according to manufacturer\u2019s instructions and calibrated against known reference materials.\u00a0 In situ salinity at the same depth was measured using a YSI Pro30.
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Turbidity:\u00a0Turbidity was measured in duplicate on discrete samples using a calibrated handheld turbidimeter (Orion AQ4500).
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Dissolved\u00a0Oxygen: Oxygen was measured optically\u00a0in situ\u00a0and atmospheric pressure measured near the sea surface using a calibrated probe (YSI ProODO) using manufactures recommendations.
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Chlorophyll: Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Methods described in Johnson et al. 2010: Chlorophyll concentrations were measured by filtering 25\u00a0 mL of seawater sample onto a 0.22 \u00b5m pore size polycarbonate filter using gentle vacuum (<100 mm Hg) and extracting in 100% MeOH at -20\u00b0C in the dark for >24 h following (Holm-Hansen and Riemann, 1978).\u00a0 Fluorescence was measured using a Turner Designs 10-AU fluorometer following (Welschmeyer, 1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2008).
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Bacteria:\u00a0Bacterioplankton (i.e. \u2018bacteria\u2019) were enumerated using a FACSCalibur flow cytometer (Becton Dickinson) and populations characterized as previously described (Johnson et al., 2010).\u00a0 Briefly, cells were excited with a 488 nm laser (15 mW Ar) and inelastic forward (<15\u00b0) scatter, inelastic side (90\u00b0) scatter (SSC), green (530 \u00b1 30 nm) fluorescence, orange fluorescence (585 \u00b1 42 nm), and red fluorescence (> 670 nm) emissions were measured.\u00a0 Bacterioplankton were quantified by staining the samples with the nucleic acid stain SYBR Green \u2013I (Molecular Probes Inc.) (Marie et al., 1997).
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Nutrients: Water was filtered through a 0.22 \u00b5m Sterivex cartridge filter,\u00a0 Millipore #SVGPL10RC using a peristaltic pump input line at 1 m for later nutrient analysis (NO3, NO2, PO4, SiOH4)\u00a0 and water was placed into duplicate HCl-cleaned HDPE bottles (VWR#414004-110) and stored at -80\u00b0C until later analysis using an Astoria-Pacific A2 autoanalyzer following the manufacturer\u2019s recommended protocols running each replicate sample in duplicate. \u00a0
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Certified reference materials were used to verify protocols (Inorganic Ventures: QCP-NT, QCP-NUT-1, CGSI1-1).\u00a0 The detection limit was NO2\u00a0= 0.05 \u00b5M, NO3\u00a0= 0.1 \u00b5M, PO4\u00a0= 0.05 \u00b5M, SiOH4\u00a0= 0.2 \u00b5M).\u00a0 Values measured below these limits are reported as zero.
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Temperature:\u00a0Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Temperature was measured in duplicate using NIST traceable thermocouples (VWR#23609-232).\u00a0 In situ water temperature at the same depth was measured using a YSI Pro30. \u00a0
Quality Scores (Q) as follows: 1=excellent (no known issues), 2=suspect, 3=poor (known reason to suspect data)
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Nutrients:\u00a0Samples that had a mean concentration (mean of replicated samples) below the nominal detection limit are reported as zero. \u00a0
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Bacteria:\u00a0Cells counts were normalized to volume sampled to determined cells per mL.
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Chlorophyll:\u00a0>0.22 um referred to as \u201ctotal\u201d or simply \u201cchlorophyll\u201d
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BCO-DMO Data Processing Notes:
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- replaced NaN with nd
\n- added ISO DateTime column
\n- separated date and time into two columns