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These data were collected by placing each strain in a 100 mL serum vial with 6 mL of their standard, published media with 30 mg zero valent iron as a source of Fe(II).\u00a0 The headspace was filled with a gas mix of 8% oxygen/10% carbon dioxide/82% nitrogen by using bottled gas mixes and a regulator to flush the headspace without over pressurization.\u00a0 Prior to sealing the serum vials, a\u00a0Presens\u00a0OPTODE dot (sensor) was placed inside the vial, allowing non-invasive gas sampling of the changes in O2 in the headspace.\u00a0 A\u00a0Presens\u00a0four channel system was used to measure changes in oxygen concentration in real-time in each bottle.\u00a0 A total of four channels were measured during each experiment: channels 1 through 3 are the biological treatments and channel 4 was a\u00a0kill\u00a0control (microbes were by placing on a heat block at 100 degrees C for 5 minutes). After 3 days of incubation, the concentration of Fe(II) in the media was measured by ferrozine assay. Old media was then removed and replaced with fresh media containing 30 ng/ml kanamycin to prevent\u00a0growth\u00a0of remaining cells. The headspace was again filled with the same gas mix and oxygen concentrations were measured in real-time in each vial. Fe(II) concentration was determined daily by ferrozine assay for 3 more days.
In these experiments, oxygen consumption rates were measured\u00a0for the iron-oxidizing bacteria (FeOB)\u00a0Mariprofundus ferrooxydans PV-1 as well as the neutrophilic FeOB \"TAG-1\" (An isolate from hydrothermal vents in the Mid Atlantic ridge that has not yet been formally named. TAG simply refers to the TAG hydrothermal site from which this microbe was isolated.). These experiments measure oxygen consumption at 100 uM oxygen at 20 degrees C. After 3 days the media in each vessel was replaced with fresh media containing kanamycin to stop any further growth and examine abiotic effects of iron mat production on zero valent iron dissolution.
Data was acquired using PreSens Measurement Studio 2 RC4 v0.5.6039.20506
\nOxygen concentration readings cut out for PV-1 after approximately 4 days - will be fixed in future experiment.
\nBCO-DMO Data Processing Notes:
\n-Reformatted column names to comply with BCO-DMO standards
\n-Reformatted dates from mm/dd/yy to yyyy/mm/dd
\n-Replaced spaces with underscores
\n-Replaced N/A with nd