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We caught wild juvenile sheepshead minnows (Cyprinodon\u00a0variegatus) from South Carolina (SC), Maryland (MD) and Connecticut (CT) in mid-August in 2014. All fish were transferred to acclimation aquaria at 24 deg C at the NOAA Fisheries Science Center, Santa Cruz, California. These temperatures represent the range experienced by sheepshead minnows from SC,\u00a0MD\u00a0and CT during a normal non-breeding season. Daily care followed standard protocols (Cripe et al. 2009, Salinas and Munch 2012), including ad libitum feeding of TetraMin flakes (Tetra Holding, Blacksburg, VA, USA). Salinity was maintained at 20\u00a0ppt,\u00a0but was reduced to 10 ppt for two days prior to egg collection. The photoperiod was 14L:10D. Each day we changed 10% of the total volume of water.
\nFor the experiments of thermal transgenerational plasticity, all eggs were divided in half and transferred to either same temperature with parent or different temperature with parent: for example, if we collected eggs from 26 deg C parents, then a half of eggs were at 26 deg C and another half of eggs were at 32 deg C. Upon hatching we randomly selected up to four larvae from each treatment group. We measured standard body length from photographs of the fish obtained with a Canon 40D digital camera with Image J (Rasband 2016). At the end of\u00a0experiment, we measured wet-mass, and\u00a0them\u00a0removed and\u00a0weighted\u00a0the testes and gonad.
Offspring growth rate from the parental contribution experiment.
Growth rate was calculated as the difference in length at 8 weeks after hatching and length at 2 weeks post-hatching divided by time because growth was linear over this period.
\nBCO-DMO Data Processing Notes:
\n- reformatted column names to comply with BCO-DMO standards
\n- filled all blank cells with nd