Dilution batch-culture bioassay experiments were established 11 May 2011 - 15 May 2011 on the SBDOM 11 cruise using water samples collected from the upper 8m of the water column. Water was gravity-filtered from the sampling Niskin bottle through two mixed cellulose ester filters (1.2 \u00b5m and 0.2 \u00b5m). Duplicate 500 mL polycarbonate bottles were filled with 375 mL 0.2 \u00b5m filtrate (filter sterilized media) and 125 mL 1.2 \u00b5m filtrate (bacterial inoculum) collected from the same cast. Bottles were incubated in the dark at 11degrees C for 10 weeks. Samples for bacterial abundance, DOM, and community composition by DNA were collected by decanting from the bottle at specified intervals.
\nDOC was quantified by high-temperature combustion on a modified Shimadzu TOC-V. We used 1.5 \u00b5M DOC as a detection limit for these experiments.
\nBacterial abundance was measured using a BD LSRII flow cytometer with an autosampler attachment. Samples were preserved in sterile cryovials with 0.2% final concentration paraformaldehyde, then stored frozen until analysis. Samples were stained with SYBR Green I for enumeration.
\nDNA was collected from duplicate bottles combined (to conserve volume); 125 mL from each bottle was vacuum-filtered onto a 0.2 \u00b5m polyethersulfone filter and frozen. Samples were lysed in sucrose lysis buffer with 1% w/v sodium dodecyl sulfate and 0.2 mg mL-1 proteinase-K, and genomic DNA was extracted using a Qiagen DNEasy silica centrifugation kit. DNA was used to conduct multiplex amplicon pyrosequencing using primers 8F and 338R, following the protocol of Nelson et al. (2014; doi: 10.1111/1462-2920.12241). Sequence analysis was conducted in mothur (Schloss et al. 2009; doi: 10.1128/AEM.01541-09) using a non-redundant subset of the SILVA SSU Ref 16S alignment database (v111) curated as in Nelson et al. (2014).
This dataset includes results from dilution batch-culture bioassay experiments reporting bacterial abundance, growth rates, barcode sequences and DOC were from the SBDOM 11 cruise in May 2011.
DOC concentrations at timepoints 0, 3, and 7 were corrected for bacterial biomass by subtracting bacterial abundance multiplied by a standard conversion factor of 10 fg C cell-1 (Fukuda et al. 1998).
\n\u00b5: \u00b5 was calculated as the rate of change of the natural log of bacterial cell abundance during exponential growth, defined here as extending from the end of lag phase to the start of stationary phase and/or to the maximum biomass observed (with additional abundance measurements from the lag and stationary phases averaged when available to increase robustness of estimates).\u00a0 Linear growth was assumed.
\nBGE: BGE was calculated as the rate of change in bacterial cell biomass [bacterial abundance multiplied by a standard conversion factor of 10 fg C cell-1 (Fukuda et al. 1998)] divided by the slope of the DOC drawdown from experiment initiation to one week, then multiplied by 100 to format as a percent.\u00a0 BGE values > 70, or those resulting from a change in DOC of < 1.5 \u00b5M, were considered invalid.
\nBCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- blank cells were replaced by 'nd' (no data)