Plocamium cartilagineum was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.
\nCulture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20\u00b0C under saturating illumination of ~150 \u00b5moles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.
\nAreal growth: Frond pieces were placed under glass without overlap of branches adjacent to a ruler for scale and imaged using an Infinity 2 camera with attached macro lens mounted on a copy stand. Images and calibrated and surface area of fronds measured using Image J. Measurements were taken at the start and end of the trial. For all measures of growth (by surface area [adj_growth], and biomass [growth_biom] and [growth_dyn]) the calculations are specific growth rates, meaning LOG(measure@t2 / measure@t1)/(t2-t1).\u00a0For surface area measures (adj_growth) and biomass (as growth_biom), t1 is the initial measure at the start of the experiment and t2 is the final measure at the end of the experiment. For biomass (asgrowth_dyn), t1 is the measure at the start of each weeklong interval and t2 is the measure at the end of the interval. Thus, there were typically 3 such intervals during a trial in which growth was measured and the datum in the column growth_dyn represents the average of those interval specific measures over the course of a trial.
\nNote: Since tagging individual fronds compromised frond performance, all fronds in a culture pot were treated as sub-samples that were summed to give the datum for the culture pot that was treated as the replicate.
\nBiomass: Frond pieces were gently blotted dry with paper toweling to remove surface water and weighed at weekly intervals to estimate biomass specific growth rates (growth_biom and growth_dyn). Note: since tagging individual fronds compromised frond performance, all fronds in a culture pot were treated as sub-samples that were summed to give the datum for the culture pot that was treated as the replicate.
\nNote: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.
\nSee Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).
This dataset includes measurements of frond area of Plocamium cartilagineum grown under various temperatures and CO2 levels. Biomass-specific growth and dynamic average of weekly growth is also calculated.
\nRelated Datasets:
\nPlocamium carbon nitrogen and stable isotopes: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)
Plocamium culture carbonate chemistry: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)
\nPlocamium culture: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)
\nPlocamium cultures pH and temperature: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)
\nPlocamium exptl treatments summary: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)
\nPlocamium pigments: Photosynthetic pigment concentrations in Plocamium cartilagineum, trials 3-8, 2014-2015 (Seaweed OA Resilience project)
\nPlocamium: pH drift: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)
\nRapid Light Curves_PAM: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions
BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- combined data from 3 submitted files: CO2trt_x_pot_trial.csv, Plo_areal_growth_CO2_Temp.csv, Plo_bio_growth_CO2_Temp.csv
\n- added columns for date_start and date_end; moved\u00a0trial\u00a0to\u00a0first\u00a0column
\n-\u00a0reduced precision of area and growth columns from 7 to 2 decimal places and adj_growth from 9 to 5 places.