Plocamium cartilagineum was collected from Santa Catalina Island and Leo Carollo, CA in June - Nov. 2014 and Jan. 2015.
\nCarbonate chemistry parameters were measured by sampling pH and total alkalinity (TA) of water samples. Seawater samples from each culture pot during all 7 trials were collected approximately every other day in 50 ml Falcon tubes to verify experimental treatment conditions. Water samples were measured usually within 1-2 hours of sample collection. pH was determined using the m-cresol indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples were analyzed by potentiometric titration coupled to a pH electrode calibrated using certified reference material (CRM) from the Dickson laboratory at Scripps Oceanographic Institute and the pH electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from potentiometric titration data and spectrophotometric pH data.
\nNote: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.
\nSee Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).
This dataset includes carbonate chemistry measurements in the Plocamium.cartilagineum growth chambers.
\nRelated Datasets:
\nPlocamium carbon nitrogen and stable isotopes: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)
Plocamium culture: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)
\nPlocamium cultures pH and temperature: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)
\nPlocamium exptl treatments summary: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)
\nPlocamium growth and biomass: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)
\nPlocamium pigments: Photosynthetic pigment concentrations in Plocamium cartilagineum, trials 3-8, 2014-2015 (Seaweed OA Resilience project)
\nPlocamium: pH drift: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)
\nRapid Light Curves_PAM: Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions
Carbonate chemistry parameters (CO2 concentration, CO2 partial pressure, CO2 fugacity, HCO3, CO3, DIC, Omega Aragonite, Omega Calcite) and total scale pH at in situ temperature were calculated using the seacarb package (V3.0.14) in R (Lavigne et al. 2011).\u00a0
\nBCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- combined data from 8 separate trial files
\n-\u00a0\u00a0reduced precision to meet sampling precision methods