Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at 15\u00b0 C under saturating illumination of ~550 \u00b5moles photons/m^2/s on a 12:12 L:D cycle. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.
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Carbonate chemistry parameters were measured by sampling pH and total alkalinity (TA) of water samples. Seawater samples from each culture pot during all 3 trials were collected 2-3 times per week in 50 ml Falcon tubes to monitor the average and variability of experimental treatment conditions. Water samples were measured usually within 1-2 hours of sample collection. pH was determined using the m-cresol indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples were analyzed by potentiometric titration coupled to a pH electrode calibrated using certified reference material (CRM) from the Dickson laboratory at Scripps Oceanographic Institute and the pH electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from potentiometric titration data and spectrophotometric pH data.
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Carbonate chemistry parameters (CO2 concentration, CO2 partial pressure, CO2 fugacity, HCO3, CO3, DIC, Omega Aragonite, Omega Calcite) and total scale pH at in situ temperature were calculated using the seacarb package (V3.0.14) in R (Lavigne et al. 2011).
This dataset reports the carbonate chemistry along with temperature, salinity and pH\u00a0of Ulva lactuca\u00a0grown in closed culture pots at varying pCO2 levels.
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Related Datasets:
\nUlva: Chl a: Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
\nUlva: CHN and stable isotopes: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)
\nUlva: Growth: Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
\nUlva: pH and temperature time-series: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)
\nUlva: pH Drift: Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)
\nUlva: Photosynthesis and respiration: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
\nUlva: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)
BCO-DMO Processing Notes:
\n- added a conventional header with dataset\u00a0name and description, PI names, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- reformatted date from m/d/yyyy to yyyy-mm-dd