Growth rate was measured in batch culture by pooling all plant thalli and summing them to attain the datum for the culture pot. Hence, the culture pot is the unit of replication. Growth rate was measured by recording the fresh weight of living material approximately every 6 days. Plant tissue was lightly stacked and gently pressed between layers of Kimwipes. This method reduced handling time and removed superficial water prior to weighing. The stack was then weighed together to attain the fresh weight for the pot. Relative growth rate was then calculated following L\u00fcning (1990):\u00a0 RGR(% . day-1 = (100*Ln(W2/W1))/(t2-t1), where W1, W2, t1, t2 correspond to fresh weights at times 1 (t1) and 2 (t2), respectively. The weekly relative growth rates track the dynamics of growth and its variation during the experiment. In addition to growth rates estimated approximately weekly, estimates of overall net and absolute growth rates integrated across the 3-week experiment were also made using the initial and final starting masses. The difference between the overall net and absolute growth rate estimates is that the latter estimate includes small tissue fragments derived from whole thalli that occasionally appeared at the end of a weekly growth interval, but were thought to not be healthy viable fragments that would generate new growth in the subsequent interval so were discarded at the beginning of the next growth interval.
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Note: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection
This dataset includes growth rates of Ulva measured at three weekly intervals as well as integrated over the experimental trial grown in culture under various pCO2 levels from May through July 2015.
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Related Datasets:
\nUlva: Carbonate chemistry pCO2: Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
\nUlva: Chl a: Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)
\nUlva: CHN and stable isotopes: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)
\nUlva: pH and temperature time-series: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)
\nUlva: pH Drift: Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)
\nUlva: Photosynthesis and respiration: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)
\nUlva: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)
BCO-DMO Processing Notes:
\n- added a conventional header with dataset name and description, PI names, version date