Symbiodinium culture preparation: Cultures were obtained from the LaJeunesse algal collection at Penn State University. Phylogenetic relationships of these strains are described in Parkinson et al., (2015). Symbiodinium cultures were grown and maintained in liquid media (ASPA-8A, Blank, 1987) at 26 \u02daC with fluorescent lights delivering 80-100 \u03bcmol quanta m-2 s-1 (measured using a DIVING-PAM equipped with a flat cosine-corrected Fiber Quantum Sensor, Walz, Germany) on a 12:12 hours Light:Dark photoperiod in Innovator 44 incubators (New Brunswick, USA). Symbiodinium cultures of equal starting concentration (3 x 104 cells 126 mL-1) for each strain were grown for 7.5 days at 26 \u02daC at logarithmic growth phase prior to experimentation. After 7.5 days of incubation at 26 \u02daC, one culture of each strain was subjected to a high temperature treatment of 31 \u02daC for two light cycles and one dark cycle (i.e., 1.5 d), whilst another was maintained at 26 \u02daC.
\nData collection:\u00a0100 \u03bcL aliquots (8.33 x 104 cells mL-1, calculated as the average of twelve independent hemocytometer measurements) of dark-adapted cultures were then subjected to 5 minutes of elevated temperatures in a thermocycler (Eppendorf Mastercycler Pro S).
\nChlorophyll fluorescence parameters were measured immediately after the 5 min temperature exposure using a Fluorometer (see below). Measurements were performed at 25 temperatures (i.e. 26, 30.3, 30.5, 31, 31.7, 32.6, 33.7, 34.8, 35.9, 36.8, 37.6, 38.1, 38.3, 38.8, 38.9, 39.4, 40.1, 41,42, 43, 44, 44.9, 45.6, 46.1, 46.2 \u02daC) and replicated three times per Symbiodinium strain per treatment. A modified protocol by D\u00edaz-Almeyda et al., 2011 was used.
\nFv/Fm was automatically calculated/determined by fitting each fluorescence transient to the bio-physical model of Kolber et al. (1998) using the FIREPRO software (Satlantic, Version 1.4.3) integrated with the FIRe system.
\nReference excitation profile used by FIReView to normalize the variable fluorescence profile: EXCFOP.TXT
\nColumn 1: incremental sample counter
\nColumn 2: elapsed time measured in microSeconds (uS)
\nColumn 3: reference excitation profile
\nColumn 4: relative fluorescence profile normalized to the reference excitation, or the fluorescence yield
\nCalibration date: 1/ 1/2002
\nTime (in seconds) of day reading was taken =\u00a079931
Methodology Reference:
\nD\u00edaz-Almeyda, E., Thom\u00e9, P. E., Hafidi, M. El, and Iglesias-Prieto, R. (2011). Differential stability of photosynthetic membranes and fatty acid composition at elevated temperature in Symbiodinium. Coral Reefs, 30(1), 217\u2013225. https://doi.org/10.1007/s00338-010-0691-5.
This dataset includes the maximum quantum yield (Fv/Fm) of clade A and B Symbiodinium cultures exposed to a range of temperatures.
\nThese data are presented in\u00a0Mansour et al., 2018.
BCO-DMO Processing Notes:
\n- added a\u00a0conventional header with dataset name and description, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions