Plocamium cartilagineum was collected from Catalina Island in June - Nov. 2014 and Jan. 2015.
\nCulture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at either 15 or 20\u00b0C under saturating illumination of ~150 \u00b5moles photons/m^2/s. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.
\nMeasurements of\u00a0chlorophyll fluorescence of photosystem II were made using a pulse amplitude modulated (PAM) fluorometer in situ in culture pots. All samples were dark-adapted prior to measurement by placing a leaf clip in the closed position over the area to be sampled for 5 minutes prior to measurement. For fluorescence measurements, the fiber optic probe was placed directly on the leaf clip perpendicular to the sample. The leaf clip was then opened and a rapid light curve program function was initiated to measure fluorescence at each of 9 photon flux densities (PFD\u2019s). PFD\u2019s of the light curve generated by the fluorometer were calibrated using an external LiCor light meter. Following measurement, the leaf clip was removed and the sample remained in the culture chamber.\u00a0
\nData were downloaded from the Diving PAM using WinControl version 3 software for Diving PAM.
\nNote: Trial 1 was a pilot test of culture system and methodological procedures so was not used for data collection in the testing of hypotheses.
\nSee Supplemental Files for a table of culture conditions for each of the 8 trials (pdf).
This dataset includes photosynthetic pigment concentrations in Plocamium cartilagineum grown under various temperatures and CO2 levels, from July 2014 to February 2015. The parameters reported are: the concentrations of Chlorophyll a, Phycoerythrin, Phycocyanin, Allophycocyanin, and total Phycobiliprotein, and the ratio of total Phycobiliprotein to Chlorophyll A.
\nRelated Datasets:
\nPlocamium carbon nitrogen and stable isotopes: Plocamium carbon and nitrogen content and stable isotope values, 2014-2015 (Seaweed OA Resilience project)
Plocamium culture carbonate chemistry: Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)
\nPlocamium culture: seawater delta13C: Stable isotope ratio and concentration of carbon in seawater during Plocamium culture experiments, 2014-2015 (Seaweed OA Resilience project)
\nPlocamium cultures pH and temperature: Plocamium culture pot pH and temperature time-series at 10 minute sampling intervals from 2014-2015 (Seaweed OA Resilience project)
\nPlocamium exptl treatments summary: Summary of pCO2 and temperature treatment combinations for each culture pot and experimental trial (Seaweed OA Resilience project)
\nPlocamium growth and biomass: Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project)
\nPlocamium: pH drift: Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)
BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- re-formatted date from dd.mm.yy to yyyy-mm-dd
\n- reordered columns to correspond with good database practices