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For APA analysis, triplicate biological samples (250 mL) from in situ and incubation samples were filtered onto 47-mm polycarbonate membranes (0.2 \u03bcm). Stored at \u221220\u00b0C until analysis.

\n

APA was assayed after Dyhrman and Ruttenberg (2006) using the fluorogenic phosphatase substrate 6,8-difluoro-4-methylumbelliferyl phosphate. Values were normalized to both volume and chl a. Reagents/Abs/Em used:

\n

D-6567 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP):
\n- Storage upon receipt: \u2264 20\u00b0C; Desiccate
\n- Abs/Em = 358/455
\n- Molecular Formula: C10H7F2O6P
\n- Molecular Weight: 292.1
\n- CAS Name/Number: 2H-1-Benzopyran-2-one, 6,8-difluoro-4-methyl-7-(phosphonooxy)-/ 214491-43-7

\n

D-6566 6,8-difluoro-7-Hydroxy-4-Methylcoumarin (DiFMU) - Reference Standard:
\n- Storage upon receipt: Room temp.; protect from light
\n- Molecular Formula: C10H6F2O3
\n- Molecular Weight: 212.15
\n- CAS Name/Number: 2H-1-Benzopyran-2-one, 6,8-difluoro-7-hydroxy-4-methyl-/ 215868-23-8

\n

Incubation key:
\nControl = no addition of nutrients or deep water
\nDSW = deep seawater addition (added 20% deep seawater (700 m))
\n+P = Added phosphate only (0.5 \u00b5M final for incubations 1 and 2, 1 \u00b5M final for incubation 3)
\n+N = Added nitrate only (6 \u00b5M final for incubations 1 and 2, 12 \u00b5M final for incubation 3)
\nphi_P = All but P added (N, Si, Fe, B12)
\n\u00a0phi_N = All but N added (P, Si, Fe, B12)
\n-1, -2, -3 = biological replicates

\n

In situ key:
\nIS = in situ
\n-1, -2, -3 = biological replicates

\n

Lost = sample was lost

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This dataset reports alkaline phosphatase activities (APA) for 3 incubation runs and 33 in situ samples collected on RV/Atlantic Explorer cruise AE1812 in May 2018. The samples were collected between Bermuda and Rhode Island.

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BCO-DMO Processing:
\n-combined 3 incubations and in situ data into one dataset.
\n-decreased number of decimal places for APA from various to 3.
\n-changed date format from m/d/yyyy to yyyy-mm-dd
\n-changed sample name phi symbol to text

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