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We collected 10 ovigerous females with stage-1 non-eyed eggs by hand and from each habitat (mangrove, salt marsh, dock) during the week preceding the full moon of each of five consecutive months throughout the A. pisonii reproductive season. Crabs were immediately placed on dry ice and stored at -80oC until dissection at which time the size and gut-width were determined and the whole egg clutch was carefully removed from the pleopods. The egg clutch was then freeze-fried and stored at -80oC until analysis.

\n

We used a modified Folch Extraction to extract the lipids from a subset (~20-40mg) of each clutch.

\n

We used a subset (~10mg) of each clutch to compare the glycogen content of eggs between habitats. We did so using a Sigma-Aldrich Glycogen Assay Kit MAK016 and determined the glycogen concentration as a percentage of egg mass through colorimetry per the manufacturer instructions.

\n

Mass of extracted lipids was calculated by subtracting the mass of the vial in which lipids was extracted from the combined mass of the lipids and the vial.

\n

Glycogen concentration was calculated using colorimetry per the manufacturer instructions.

\n

Locations, Florida East Coast:
\nRound Island Park: 27o33'33\"N 80o19'53\"W
\nPepper Park: 27o29'42'N 80o18'12\"W
\nNorth Causeway Park: 27o28'28\"N 80o19'12\"W
\nOslo Road: 27o35'14\"N 80o21'55\"W
\nAnastasia State Park: 29o52'40\"N 81o16'32\"W
\nGuana-Tolomato-Matanzas NERR: 30o0'49\"N 81o20'42\"W
\nVilano Inlet: 29o55'16\"N 81o17'57\"W
\nPalm Valley/Nocatee Canoe Launch: 30o07'57\"N 81o23'08\"W
\nSt. Augustine Yacht Club: 29o53'09\"N 81o17'08\"W
\nBoating Club Road: 29o56'34\"N 81o18'31\"W

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This dataset includes calculations of the lipid and glycogen content of\u00a0eggs of mangrove tree crabs, A. pisonii, from three habitats: mangrove, saltmarsh and mangrove collected along the eastern Florida coast in 2016.

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BCO-DMO Processing:
\n- added ISO_Date columns; removed Month (number) and Date (d-Mon) column from display
\n-\u00a0reduced decimal precision of: GW_CW from 9 to 3 places; Lipid_wt, Glycogen_Conc, Glycogen_pct (9 to 5)
\n- changed Glycogen_perc to Glycogen_pct to match Lipid_pct

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