We collected 10 ovigerous females with stage-1 non-eyed eggs by hand and from each habitat (mangrove, salt marsh, dock) during the week preceding the full moon of each of five consecutive months throughout the A. pisonii reproductive season. Crabs were immediately placed on dry ice and stored at -80oC until dissection at which time the size and gut-width were determined and the whole egg clutch was carefully removed from the pleopods. The egg clutch was then freeze-fried and stored at -80oC until analysis.
\nLipids were extracted from eggs using a modified Folch extraction and the fatty acids were then methylated. We then analyzed the methylated fatty acids via gas chromatography-mass spectrometry using an Agilent 6890 GC 5975 Mass Spectrometer fitted with a Restek 30m FAMEWAX column. The concentration of each FA was then determined from a diluton curve derived from a Supelco 37 Fatty Acid mix. The FA peak areas were first normalized to recovery standard then concentrations were determined using the regression curves of the external standards. This concentration was then corrected for the volume of the sample to obtain the mass of the fatty acid extracted from the egg.
\nGut-width:carapace-width ratio was calculated as the ratio of the size of the gut of the crab and the size of the crab.
\nThe mass of Omega-3 Fatty Acids was determined by combining the masses of Alpha Linoleic Acid, Eicosatrienoic Acid, Eicosapentaenoic Acid, and Docosahexaenoic Acid.
\nThe mass of Omega-6 Fatty Acids was determined by combining the masses of cis-Linoleic Acid, trans-Linoleic Acid, Eicosadienoic Acid, Dihomo-Gamma-Linolenic Acid, Arachidonic Acid, and Docosahexaenoic Acid.
\nThe mass of the highly-unsaturated Fatty Acids was determined by combining the masses of Eicosapentaenoic Acid, Arachidonic Acid, and Docosahexaenoic Acid.
\nThe mass of odd-numbered Fatty Acids was determined by combining the masses of C15:0 fatty acid, C15:1 fatty acid, C17:0 fatty acid, and C17:1 fatty acid.
\nThe mass of each fatty acid extracted from the eggs was divided by the mass of the eggs from which fatty acids were extracted to obtain the concentration of that fatty acid in the eggs as the proportion of the egg mass.
\nLocations, Florida East Coast:
\nRound Island Park: 27o33'33\"N 80o19'53\"W
\nPepper Park: 27o29'42'N 80o18'12\"W
\nNorth Causeway Park: 27o28'28\"N 80o19'12\"W
\nOslo Road: 27o35'14\"N 80o21'55\"W
\nAnastasia State Park: 29o52'40\"N 81o16'32\"W
\nGuana-Tolomato-Matanzas NERR: 30o0'49\"N 81o20'42\"W
\nVilano Inlet: 29o55'16\"N 81o17'57\"W
\nPalm Valley/Nocatee Canoe Launch: 30o07'57\"N 81o23'08\"W
\nSt. Augustine Yacht Club: 29o53'09\"N 81o17'08\"W
\nBoating Club Road: 29o56'34\"N 81o18'31\"W
This dataset includes concentrations of fatty acids from eggs of mangrove tree crabs, A. pisonii, from three habitats: mangrove, saltmarsh and mangrove collected along the eastern Florida coast in 2016.
BCO-DMO Processing:
\n-\u00a0reduced decimal precision of: GW_CW from 9 to 3 places