Samples were collected manually by filling an acid-washed and autoclaved 20L carboy after three rinses. Temperature, pH, and salinity were taken using a handheld YSI. Cell counts were obtained by filtering water through a 2.7 \u00b5m Whatman GF/D filter, fixing with 10% formaldehyde, placing on ice, and then counting using flow cytometry (Thrash et al., 2015, Hydrocarbon and Lipid Microbiology Protocols). Inorganic nutrients were measured at the University of Washington Marine Chemistry Laboratory after sequential filtration through 2.7 Whatman GF/D and 0.22 \u00b5m Sterivex filters. Samples were initially placed on ice in the field, and then refrigerated until shipment with ice packs.
Data Problem Report:\u00a0
\npH is not reported for the first time points because a crack was noticed in the housing of the YSI cable that seemed to be associated with calibration. This was subsequently replaced and all values were also checked in the lab with a Fisherbrand pH meter.
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Sample ARD 9/19/18 was not filtered on site because of bad weather. Therefore, the filtration steps for nutrients were conducted back at the lab after a 2.5 hour transit time
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Sample ARD 11/16/18 was filtered for nutrients after a 3 hour delay at site CJ because of power supply issues at the ARD site.
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BCO-DMO Data Processing Notes:
\n-Reformatted column names\u00a0to comply with BCO-DMO standards;
\n-Replaced blank cells with nd;
\n-Reformatted dates;
\n-Added ISO DateTime column.