Spawning-ripe sand lance were collected from SBNMS (42\u00b0 9' 58.26'' N, 70\u00b0 18' 44.19'' W) on 2 December 2016 and 22 November 2017, using a 1.3 \u00d7 0.7 m beam trawl (6 mm mesh) towed at 3 knots for 15 minutes.
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We tested factorial combinations of three pCO2 \u00d7 three temperature levels. The target for pCO2 controls was 400 \u00b5atm (~8.15 pHNIST), a level characteristic of the average open ocean and of the A. dubius spawning habitat in late fall (Salisbury and J\u00f6nsson, 2018). As contrasts, we chose 1,000 \u00b5atm (~7.78 pH) and 2,100 \u00b5atm (~7.48 pH), which correspond to predicted average ocean pCO2 levels by the years 2100 and 2300, respectively.
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Response traits: Treatment-specific embryo survival was calculated in trials 1 and 3 as the proportion of hatchlings relative to the number of fertilized embryos (%), while in trial 2 the total number of hatchlings was used as a proxy for embryo survival. In trial 3, fertilization success (%) was calculated as the sum of hatched larvae + arrested embryos divided by 300. Treatment-specific daily hatch frequencies (= daily treatment hatch/total hatch within temperature treatment) were calculated for trials 2 and 3.
Data processing: Data were cross-checked with hand-written notes, checked for outliers/erroneous entries/numbers.
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BCO-DMO Data Processing:
\n-\u00a0modified parameter names (replaced spaces with underscores, replaced \"%\" with \"pcnt\")
\n-\u00a0converted latitude and longitude from degrees, minutes, seconds to decimal degrees
\n-\u00a0reformatted date from mm/dd/yyyy to yyyy-mm-dd
\n-\u00a0replaced \"n/a\" with \"nd\" (no data);
\n- replaced earlier copy of data (2018-11-07)\u00a0with revised copy on 2019-09-20;
\n- version 2 published on 2021-05-27. This version contains corrections to the\u00a0pcnt_hatching_success data values.