Water samples for chlorophyll extraction were collected either from the mesocosms via a 5 L Niskin or subsampled from experimental bottles. Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in the dark at room temperature. After extraction, filters were removed from the sample, and the fluorescence of the sample was read on a Turner AU10. The sample was then acidified with 1 drop of 10% HCL and re-read on the same instrument. The fluorometer was calibrated prior to using with a chlorophyll standard purchased from Sigma.\u00a0
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Mesocosm treatment for all HHQ experiments was as follows:
\nRedfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading
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HHQ treatments here are as follows:
\nHigh HHQ - 100 ng mL-1 (410 uM) added to triplicate 5L bottles.
\nDMSO control - equivalent (v:v) DMSO added to triplicate 5L bottles.
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\u00a0All bottles were incubated for 24h in a flow-through tank, that was shaded to mimic in situ conditions. Chlorophyll samples were taken at T0 and T24 for all experiments.
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Data were processed in Excel with statistics run in Excel, R, or Matlab.
This dataset includes chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway. Microbial mesocosms were spiked with 2-heptyl-4-quinolone (HHQ).
BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- reduced precision of total chlorophyll columns from (5 to 15) to 2 decimal places