Colonies that displayed a strong bleaching phenotype in April 2015 were selected for transcriptome-wide gene expression analyses. These colonies were subsequently sampled in August 2015, December 2015 and April 2016. For these 36 field-collected tissue samples (five colonies of A. gemmifera and four colonies of A. hyacinthus across four sample dates), total RNA was extracted Qiagens RNAeasy Plus Kit. In total 36 cDNA libraries were generated using the Illumina TruSeq RNA Library Prep Kit v2 with Protoscript II Reverse Transcriptase. We carried out multiplexed Illumina sequencing at the University of Utah Microarray and Genomic Analysis Core Facility. Fastq files were mapped to a reference transcriptome (Barshis et al., 2013) using HISAT2 (Langmead & Salzberg, 2012) with a minimum mapping quality of 10. We used SAMtools (Li et al., 2009) to generate counts for each contig in our reference transcriptome. Counts matrices were normalized in DESeq2.0 (Love, Huber, & Anders, 2014).
\nApproximate coordinates for this dataset are "Pool 400", back reef lagoon, Ofu, American Samoa (-14.17990, -169.65448)
This dataset includes accession numbers for 36 RNAseq libraries housed at\u00a0The National Center for Biotechnology Information (NCBI).\u00a0 Coral colonies that displayed a strong bleaching phenotype at Ofu Island, American Samoa were sampled between 2015 and 2016.\u00a0
\nThe genetic accessions at NCBI referenced in this dataset will not be publicly accessible until\u00a02019-05-01.\u00a0 \u00a0This includes accession numbers and links to the accession page.
\nThese data were published in Thomas & Palumbi (2017).\u00a0 \u00a0
BCO-DMO Data Manager Processing Notes:
\n* added a conventional header with dataset name, PI name, version date
\n* modified parameter names to conform with BCO-DMO naming conventions
\n* added column of links to genetic accessions at NCBI
\n* Added separate year and month columns from parsing the Date column (format yyyy_mm )