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Alvin pushcores dedicated to nutrient analyses were sectioned to recover the 0-6cm, 6-12, and 12-18cm fractions, or the 0-10, 10-20, and 20-30cm fractions. Two ~40ml samples of sediment were placed in 50 ml Falcon tubes and were centrifuged at 3000 rpm for 15 minutes to separate porewater from the sediment. After centrifugation, the porewater was filtered through sterile syringe cellulose 0.45 micron filters. 5 ml of the porewater was placed into 5 ml screwcap Eppendorf cryotubes stored without headspace at -20 degrees C for nitrate (NO3), nitrite (NO2) and phosphate (PO4) analysis. The headspace was flushed with nitrogen and the samples were stored inverted at 4 degrees C.
\nAll porewater analyses were performed at Louisiana State University Wetland Biogeochemistry Analytical Services. Dilution info: All samples @ 2x and various samples @ 20x , 50x , 70x , and/or 350x.
Porewater nutrient concentrations from pushcore samples collected at two locations in the Guaymas Basin hydrothermal vents region during Alvin dives on RV/Atlantis cruise AT42-05, Nov. 2018. NO3+NO2, NH4, and PO4 concentrations at different depths within the cores are reported. These samples are reported by the PI at\u00a0Woods Hole Oceanographic Institution.
\nNutrient porewater samples from this cruise were also analyzed at the Max Planck Institute for Marine Microbiology. See related dataset: https://www.bco-dmo.org/dataset/816549 (MPI)
BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- re-formatted date from d/m/yyyy to yyyy-mm-dd