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For each temperature run (i.e., each experimental level) eight urchins of each species were collected from Cerro Tijieretas cove (0\u00b053\u201916.78\u201dS, 89\u00b036\u201929.18\u201dW) at an average depth of 4 m. Urchins were transported in plastic containers filled with sea water to the UNC/USFQ Galapagos Science Center.\u00a0
\nEach individual, whose volume has been calculated using a beaker (ie, via water displacement), was randomly assigned to a numbered mesocosm: green and pencil urchins were placed in 21x11x9 cm and 42x9 cm (circumference x depth), respectively, with 5g (wet weight) of fresh Ulva sp. each taken from the Cerro Tijieretas site. Ulva sp. was weighed after excess water was removed using a salad spinner that was cycled twice for 20 repetitions. The 16 mesocosms were then placed randomly within a large water bath of 130x60x35 dimensions at the temperature to be tested randomly. A chiller and two heaters, paired with an Apex aquarium thermostat, were used to control the water temperature inside the water bath. Each mesocosm closed with a wire mesh top so that the water temperature inside the mesocosm was the same as that in the aquarium and water could be exchanged freely. Before the test, the individuals were left to fast for 24 hours in basins with oxygenators. The water inside the basins was changed approximately every 12 hours with new sea water at an ambient temperature of ~23\u00b0C. This procedure was repeated for every temperature tested.
This study was conducted from February to March 2018 at the Galapagos Science Center on San Cristobal Island. The rate of Ulva sp. consumption by the two sea urchins, Lytechinus semituberculatus and Eucidaris galapagensis was measured at 10 temperatures: 14\u00b0, 16\u00b0, 18\u00b0, 20\u00b0, 22\u00b0, 24\u00b0, 26\u00b0, 28\u00b0, 30\u00b0, 32\u00b0C. L. semituberculatus (green urchin), E. galapaguensis (pencil urchin) and T. depressus (white urchin) are the three most common species in the Galapagos Islands and together make up 91% of the sea urchin biomass.\u00a0 Ulva sp. was used as the prey item because it is one of the most abundant macroalgal species, together with turf, encrusting coralline algae and Sargassum near the Galapagos\u00a0 and\u00a0 coast and because it is highly palatable for herbivores.
BCO-DMO Processing Notes:
\n-\u00a0added conventional header with dataset name, PI name, version date