Sinking particles were collected at 1500 m and 3200 m depths by Parflux sediment traps (McLane Labs, Falmouth, MA) on the Oceanic Flux Program (OFP) mooring. Trap cups were filled with 40 ppt deep seawater (concentrated by freezing) poisoned with ultra-trace metal purity HgCl2 (200 mg L-1). OFP methods are detailed in Conte et al. (2001, 2018). Sinking particles analyzed in this study were collected between 25 October 2016 and 10 December 2016 with a biweekly sample resolution.
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Lipids were preserved and analyzed using a modification of methods in Conte et al. (2003) (Pedrosa-P\u00e0mies et al., 2018). Briefly, an internal standard mixture (n-C21:0 fatty alcohol, n-C23:0 fatty acid, 5\u03b1-cholestane and n-C36:0 alkane) was added to the samples prior to lipid extraction. Lipids were ultrasonically extracted in 2:1 CHCl3-MeOH, transesterified with methanolic HCl and trimethylsilylated using BSTFA with 1% TMCS. The transesterified, trimethylsilyl derivatives were analyzed on an Agilent 7890A GC coupled to a 5975C MS. Compounds were identified by mass spectra and quantified from their FID response relative to the internal standard.
Fluxes of lipid biomarkers in the 1500 m and 3200 m Oceanic Flux Program sediment traps in the northern Sargasso Sea, before, during and after of Hurricane Nicole 2016 (Sep-Dec 2016)
Lipid compounds were identified by their mass spectra and were quantified from their FID response relative to the internal standard. The software used were MSD ChemStation (version E.0202.1431) and Chrom Perfect (version 5.5.2).
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BCO-DMO Processing Notes:
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