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Wild adults were collected using a 30 x 2 m beach seine and strip-spawned in the laboratory the following day. 100 embryos were then placed in each replicate across 9 recirculating systems of different pCO2 x DO conditions (control, intermediate, extreme) and cycling patterns (static, small diel fluctuation, large diel fluctuation and tidal fluctuation).
\npCO2 x DO conditions were measured every hour for each tank and adjusted to the pre-determined conditions via the injection of carbon dioxide, nitrogen gas and/or CO2-stripped air.\u00a0LabView software (National Instruments) was used to control sampling pumps and gas and water solenoids.
\nNewly hatched larvae were counted every day to determine embryo survival. Surviving larvae at 6 or 15 days post hatch were counted to obtain larvae. For more details please see Cross et al. (submitted).
Survival data from static and fluctuating pCO2 x dissolved oxygen (DO) experiments on Menidia menidia. Four separate experiments were conducted over two consecutive years to determine the effects of static and fluctuating pCO2 x DO conditions on the early life survival and growth of the coastal forage fish, M. menidia.\u00a0
\nSee related datasets: growth and carbonate chemistry.
Survival data was processed using R.