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Wild adults were collected using a 30 x 2 m beach seine and strip-spawned in the laboratory the following day. 100 embryos were then placed in each replicate across 9 recirculating systems of different pCO2 x DO conditions (control, intermediate, extreme) and cycling patterns (static, small diel fluctuation, large diel fluctuation and tidal fluctuation).
\npCO2 x DO conditions were measured every hour for each tank and adjusted to the pre-determined conditions via the injection of carbon dioxide, nitrogen gas and/or CO2-stripped air.\u00a0LabView software (National Instruments) was used to control sampling pumps and gas and water solenoids.
\nSeawater samples of each tank were collected three times throughout each experiment and stored in 4\u00b0C for subsequent alkalinity measurements up to two weeks after the experiment finished. For more details please see Cross et al. (submitted).
Carbonate chemistry data from static and fluctuating pCO2 x dissolved oxygen (DO) experiments on Menidia menidia. Four separate experiments were conducted over two consecutive years to determine the effects of static and fluctuating pCO2 x DO conditions on the early life survival and growth of the coastal forage fish, M. menidia.\u00a0
\nData processing:\u00a0CO2SYS version 2.1 was used to calculate pCO2 in the carbonate chemistry tables.