Chaetoceros simplex cultures, were obtained from population strain CCMP 200 (National Center for Marine Algae and Microbiota, NCMA).
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Thermal performance curve (TPC) assays:
\nWe assayed the TPCs of our populations twice during the evolution experiment. This involved pre\u2010acclimating sub\u2010cultures from each population to 28 \u00b0C (in\u2010between the 25 \u00b0C control and 31 \u00b0C experimental treatment) in N\u2010replete medium for 20 days (20\u201325 generations) to remove any effects of acclimation to previous temperatures (31 or 25 \u00b0C) and N levels. Subsequently, separate flasks containing N\u2010replete medium were placed at each assay temperature, inoculated with pre\u2010acclimated populations, and allowed to acclimate for six more days.
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After ~ 200 generations of evolution (165\u2013186 days: 194\u2013232 generations), we characterised the TPCs of two randomly selected N\u2010replete evolved populations that were 34 \u00b0C\u2010tolerant (see the 34 \u00b0C challenge section below) and the control and an ancestral population. Assay temperatures were 10, 20, 25, 29, 31, 32, 34 and 35 \u00b0C, and three replicate populations were grown in 50\u2010mL culture flasks (instead of well plates) (96 growth rate estimates).\u00a0We measured in vivo chlorophyll\u2010a fluorescence (excitation wavelength: 436 nm, emission wavelength: 680 nm) daily using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) to estimate the growth rate.
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Growth rate calculations:
\nFrom the daily biomass estimations (in vivo chlorophyll\u2010a fluorescence ), we calculated population growth rates (day\u22121), as the slope of the linear regression of ln(biomass) vs. time (days).
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\nAlso see data for 100 generations:\u00a0https://www.bco-dmo.org/dataset/778749
\nMore details in Aranguren-Gassis et al. 2019, Ecology Letters.
Daily growth rates for Thermal Performance Curve (TPC) of Chaetoceros simplex in nitrogen-replete evolved populations after about 200 generations of evolution at eight temperatures, 10-35 degrees C.
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After ~ 200 generations of evolution (165\u2013186 days: 194\u2013232 generations), we characterised the TPCs of two randomly selected N\u2010replete evolved populations that were 34 \u00b0C\u2010tolerant (see the 34 \u00b0C challenge section below) and the control and an ancestral population. Assay temperatures were 10, 20, 25, 29, 31, 32, 34 and 35 \u00b0C, and three replicate populations were grown in 50\u2010mL culture flasks (instead of well plates) (96 growth rate estimates).
BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- reduced precision of Growth_rates from 9 to 1 decimal places.