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Complete methods are outlined in Anderson and Rynearson, 2020, in press.
\nElemental Analyses: From each culture and during exponential growth phase, >1 x 107 cells were filtered in triplicate onto precombusted 25-mm GF/F filters, and rinsed with 10 ml F/2 media. Blanks were made to correct for any elemental contribution from filters or media. Filters were dried at 60 degrees C for 24 hrs. The mass of the filter, total volume filtered, and cell counts were utilized to determine the number of cells analyzed. Elemental composition was assessed on an elemental analyzer.
\nCell volume: Linear measurements of height and diameter were recorded for 30 live cells from each strain and used to calculate cell volume using the volume of a cylinder (Montagnes and Franklin 2001).
\nAll data processing was carried out in R 3.4.1(R-Core-Team 2015).
This dataset includes carbon and nitrogen concentrations of Skeletonema cultures maintained at six temperatures. Strains were collected at Narragansett Bay, Rhode Island.
BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- re-formatted date from m/d/yyyy to yyyy-mm-dd
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