Sampling of cores was done in accordance with IODP protocols.
\nAll C0014 samples were cored on 16 September 2010 via via HPCS (hydraulic piston coring system).
\nAll C0015 samples were cored on 18 September 2010 via HPCS.
\nAll C0017 samples were cored on 27 September 2010 via HPCS.
Core sections were kept frozen (-80\u00b0C) until DNA extractions. A MoBio\u00ae Power Soil kit was used to extract environmental DNA. Metagenomic data was produced from whole genome amplified environmental DNA (REPLI-g Mini Kit, Qiagen).
\nSequencing was performed at the Penn State Genomics Core Facility \u2013 University Park, PA using the Illumina\u00ae HiSeq 2500 (NSF-MRI award DBI-1229046 (Axtell et al., 2012)). The sequencing facility prepared DNA libraries using Nextera XT Library Preparation Kits prior to sequencing. Together, the samples were run on one-half of a sequencing plate. This sequencing run was run using rapid-run model that averages up to 300 million single reads, or 600 million paired reads of ~150 nucleotides per rapid-run.
Next-generation sequencing data across a hydrothermal gradient; Okinawa Backarc Basin IODP 331 \u2013 subvent biosphere.\u00a0The purpose these metagenomic data was to capture a distinct changeover of microbial assemblages between the mesophilic horizons above 10 mbsf and the hottest (55C) hydrothermal clay horizon. In this section of the dynamic Iheya North Hydrothermal system, the temperature gradient was estimated to be ~3C/m. These metagenomes provide functional evidence to this model, demonstrating that molecular signals represent a responsive microbial community to the increasingly demanding environmental conditions.
Data Processing: Data assembly was performed with Ray Meta de novo assembly program using a k-mer of 65 for all samples.