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Natural seawater was enriched for photoautotrophs and split into multiple temperatures for two weeks. After the enrichment period, Synechococcus was isolated from each temperature. Each isolate's thermal niche was measured through a series of lab experiments and sequenced.
\n200ml of dense culture was concentrated by centrifuging 25,000 x g for 15 minutes. The subsequent cell pellet was resuspended in 5ml of sterile growth media. Cell concentrate was dark acclimated for 10 minutes and 200ul was used to measure the fluorescence spectra. After each measurement, the temperature increased and cells were dark acclimated at that temperature for an additional 10 minutes. This continued until the fluorescence decreased to ~0 indicating the disassociation of the photosynthetic apparatus. This was done following the methods published in Pittera et al., 2017.
Fluorescence spectra from 600-700nm at 530nm excitation for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature
BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
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