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A batch culture (1.5L) of Trichodesmium erythraeum sp. IMS101 was grown in a 27\u00b0C incubator with a 14:10 light cycle that ramps up and down mimicking dawn and dusk. Sampling occurred every 1-3 hours with concentrated sampling at dawn and dusk. 70mL of the culture was sterically subsampled and collected on 0.2mm Supor filters, then frozen at -80\u00b0C. 10mL was collected and filtered on combusted GFF filters for CHN analysis.
\n
\u00a0
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Proteins were extracted in sodium dodecyl sulfate and digested in gel similar to Saito et al., 2014 (Science). Peptides were analyzed by LC-MS/MS on a Thermo Orbitrap Fusion using LC x LC/MS chromatography with high and low pH reversed phase chromatography.
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Diel proteomes of cultured\u00a0Trichodesmium erythraeum\u00a0sp. IMS101 from laboratory experiments conducted in November of 2018.
\n
\u00a0
\n
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [1] partner repository with the dataset identifier PXD016332 and 10.6019/PXD016332 but are not yet public.
\n
\u00a0
\n
\u00a0Project Name: Trichodesmium erythraeum sp. IMS101 Diel proteomes
\n
\u00a0Project accession: PXD016332
\n
\u00a0Project DOI: 10.6019/PXD016332
\n
\u00a0
\n
The format of these data in the BCO-DMO data system is tabular.\u00a0 For a version formatted as a matrix, see the \"Data Files\" section.
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Peptide to spectrum matching was performed in SEQUEST implemented in Proteome Discoverer 2.2 using the Trichodesmium erythraem sp. IMS101 genome. Statistical validation was performed at the 1% protein and peptide FD levels calculated in Scaffold (Proteome Software).
\n
\u00a0
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BCO-DMO Data Manager Processing Notes:
\n
* originally submitted file \"dielproteindata.csv\" in matrix format added to \"Data Files\" section.
\n
* a tabular version of dielproteindata.csv was created and imported into the BCO-DMO data system.\u00a0 Data was unpivoted to transform from the matrix into a table with columns for hours_post_dawn,cnratio,relative_protein_abundance.
\n
* added a conventional header with dataset name, PI name, version date
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* modified parameter names to conform with BCO-DMO naming conventions (spaces, +, and - changed to underscores).\u00a0 Units in parentheses removed and added to Parameter Description metadata section.
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* blank values in this dataset are displayed as \"nd\" for \"no data.\"\u00a0 nd is the default missing data identifier in the BCO-DMO system
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