{"@context":{"content":"http://purl.org/rss/1.0/modules/content/","dc":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","og":"http://ogp.me/ns#","rdfs":"http://www.w3.org/2000/01/rdf-schema#","sioc":"http://rdfs.org/sioc/ns#","sioct":"http://rdfs.org/sioc/types#","skos":"http://www.w3.org/2004/02/skos/core#","xsd":"http://www.w3.org/2001/XMLSchema#","owl":"http://www.w3.org/2002/07/owl#","rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","rss":"http://purl.org/rss/1.0/","site":"https://www.bco-dmo.org/ns#","odo":"http://ocean-data.org/schema/","emo":"http://ocean-data.org/schema/entity-matching#","bibo":"http://purl.org/ontology/bibo/","crypto":"http://id.loc.gov/vocabulary/preservation/cryptographicHashFunctions/","bcodmo":"http://lod.bco-dmo.org/id/","tw":"http://tw.rpi.edu/schema/","dcat":"http://www.w3.org/ns/dcat#","time":"http://www.w3.org/2006/time#","geo":"http://www.w3.org/2003/01/geo/wgs84_pos#","geosparql":"http://www.opengis.net/ont/geosparql#","sf":"http://www.opengis.net/ont/sf#","void":"http://rdfs.org/ns/void#","sd":"http://www.w3.org/ns/sparql-service-description#","dctype":"http://purl.org/dc/dcmitype/","prov":"http://www.w3.org/ns/prov#","schema":"http://schema.org/","geolink":"http://schema.geolink.org/1.0/base/main#","spdx":"http://spdx.org/rdf/terms#","bcodmo_vocab":"http://schema.bco-dmo.org/"},"@id":"http://lod.bco-dmo.org/id/dataset/785167#graph","@graph":[{"http://lod.bco-dmo.org/id/dataset/785167":{"@id":"http://lod.bco-dmo.org/id/dataset/785167","@type":["http://ocean-data.org/schema/DeploymentDatasetCollection","http://www.w3.org/ns/dcat#Dataset","http://ocean-data.org/schema/Dataset"],"http://ocean-data.org/schema/hasAcquisitionDescription":[{"@value":"
Adult C. virginica (9.55 cm \u00b1 0.45) were collected from an intertidal oyster reef in Plum Island Sound, MA (42.681764, -70.813498) in mid-July 2016. The oysters were transported to the Marine Science Center at Northeastern University (Nahant, MA), where they were cleaned and randomly assigned to one of six flow-through tanks (50L) maintained at ambient seawater conditions. Oysters were acclimated for 14 days under ambient conditions, before initiating a 28-day experimental exposure. The oysters were exposed to either control (500 \u00b5atm) or elevated pCO2 (2500 \u00b5atm; \u03a9calcite < 1).
\nDNA was isolated from ten gonad tissue samples using the E.Z.N.A. Mollusc Kit (Omega) according to the manufacturer\u2019s instructions. Isolated DNA was quantified using a Qubit dsDNA BR Kit (Invitrogen). DNA samples were sonicated for ten minutes at 4 \u00baC, on 30 second intervals periods at 25% intensity. Shearing size (350bp) was verified using a 2200 TapeStation System (Agilent Technologies). Samples (10) were enriched for methylated DNA using MethylMiner kit (Invitrogen). Libraries were prepared using Pico Methyl-Seq Library Prep Kit (Cat. #D5455).
Eastern oyster gonad methylation patterns in response to experimental ocean acidification at pCO2 levels 400 and 2800 ppm. Oysters were collected from an intertidal oyster reef in Plum Island Sound, MA, Gulf of Maine in mid-July 2016. This dataset includes GenBank BioProject PRJNA513384 metadata.
BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names: filename3 to MBD_cv_id; filename4 to pCO2_treatment
\u00a0